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Series GSE118681 Query DataSets for GSE118681
Status Public on Jul 01, 2021
Title Temporal controls over interareal cortical projection neuron fate diversity (MAPseq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Other
Summary Interconnectivity between neocortical areas is critical for sensory integration and sensorimotor transformations. These functions are mediated by heterogeneous interareal cortical projection neurons (ICPN), which send axon branches to distinct cortical areas as well as to subcortical targets. Although ICPN are anatomically diverse, they are molecularly homogeneous and how the diversity of their anatomical and functional features emerge during development remains largely unknown. Here, we address this question by linking connectome and transcriptome in developing single ICPN of the mouse neocortex using a combination of MAPseq mapping (to identify single-neuron axonal projections) and single-cell RNA sequencing (to identify corresponding gene expression). Focusing on neurons of the primary somatosensory cortex (S1), we reveal a protracted unfolding of the molecular and functional differentiation of motor cortex-projecting (M) compared to secondary somatosensory cortex-projecting (S2) ICPN. We identify SOX11 as a temporally differentially expressed transcription factor in M vs. S2 ICPN. Postnatal manipulation of SOX11 expression level in S1 impaired sensorimotor connectivity and selectively disrupted exploratory behavior in freely moving mice. Together, our results reveal that within a single cortical area, different subtypes of ICPN have distinct postnatal molecular differentiation paces, which is then reflected in distinct circuit connectivities and functions. Dynamic differences in expression levels of largely generic set of genes, rather than fundamental differences in the identity of developmental genetic programs, may thus account for emergence of intra-type diversity in cortical neurons.
 
Overall design This record contains mapseq data from P14, P5, P7 mouse cortex in quadruplicate. A 32bp-barcoded sindbis virus is injected in the primary somatosensory cortex S1. The virus enter the neurons, replicate and migrate to the terminal connections. Then, S1 injection area and 6 projection targets where S1 cells are expected to project are dissected (A, C, M, S2, Sub, V). Barcodes of the Sindbis viruses are sequenced by bulk RNA-seq for each area so that we can link S1-cells to their projection targets.
 
Contributor(s) Klingler E, Tomasello U, Prados J, Kebschull J, Contestabile A, Galiñanes G, Fièvre S, Huber D, Dayer A, Bellone C, Santinha A, Platt R, Jabaudon D
Citation(s) 34754107
Submission date Aug 17, 2018
Last update date Nov 19, 2021
Contact name Julien Prados
E-mail(s) julien.prados@unige.ch
Phone +41 22 37 95 396
Organization name University of Geneva
Department SCMU
Lab Bioinformatics Support Platform
Street address Rue Michel Servet 1
City Geneva
ZIP/Postal code 1211
Country Switzerland
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (84)
GSM4676689 Sindbis reads sequenced from dissected target A in pup Q at age P14
GSM4676690 Sindbis reads sequenced from dissected Contralateral targets in pup Q at age P14
GSM4676691 Sindbis reads sequenced from dissected target M in pup Q at age P14
Relations
BioProject PRJNA486447
SRA SRP158190

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE118681_mapseq_links.tsv.gz 4.5 Mb (ftp)(http) TSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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