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| Status |
Public on Oct 10, 2008 |
| Title |
Expression data from mouse aorta-gonad-mesonephros(AGM) derived stromal cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
A mouse AGM-derived cell line, AGM-s3, was shown to support the development of hematopoietic stem cells. To elucidate the molecular mechanisms regulating early hematopoiesis, we obtained subclones from AGM-s3, some of which were hematopoiesis supportive (s3-A9) and others which were non-supportive (s3-A7), and we analyzed the gene expression profiles by gene chip analysis.
Keywords: cell type comparison
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| Overall design |
Genome-wide gene expression was examined using Affymetrix GeneChip array. Assays were performed according to the manufacturer's protocol. Total RNA was isolated from each stromal cell lines. We analysed 3 cell lines, AGM-s3-A9, AGM-s3-A7 and OP9. AGM-s3-A9 and OP9 are hematopoiesis supportive cell lines. AGM-s3-A7 is a hematopoiesis non-supportive cell line.
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| Contributor(s) |
Sohma Y, Yoneya T, Nagao K |
| Citation(s) |
18840400 |
| |
| Submission date |
Jun 26, 2008 |
| Last update date |
Feb 18, 2018 |
| Contact name |
Kenji Nagao |
| E-mail(s) |
kenji.nagao@kyowa-kirin.co.jp
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| Organization name |
Kyowa Hakko Kirin Co., Ltd.
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| Department |
Innovative Drug Research Laboratories
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| Street address |
3-6-6, Asahi-machi,
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| City |
Machida-shi |
| State/province |
Tokyo |
| ZIP/Postal code |
194-8533 |
| Country |
Japan |
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| Platforms (3) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
| GPL82 |
[MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array |
| GPL83 |
[MG_U74Cv2] Affymetrix Murine Genome U74 Version 2 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA105717 |
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