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| Status |
Public on Aug 28, 2018 |
| Title |
Reovirus infection induces stabilization and up-regulation of cellular transcripts that encode regulators of TGF-beta signaling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We utilized oligonucleotide microarrays to measure cellular mRNA decay rates in mock- or reovirus-infected murine L929 cells to determine if changes in host mRNA expression are a consequence of reovirus-induced alterations in cellular mRNA stability.
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| Overall design |
Murine L929 cells were mock-infected or infected with reovirus strains clone87, clone8, and Dearing. At 19.5 h p.i., ongoing transcription was arrested by addition of actinomycin D and global mRNA expression levels were measured after 0, 45, 90 and 120 min of actinomycin D treatment using Affymetrix U74Av2 microarrays
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| Contributor(s) |
Guo L, Smith JA, Abelson M, Vlasova-St. Louis I, Schiff LA, Bohjanen PR |
| Citation(s) |
30261045 |
| |
| Submission date |
Aug 27, 2018 |
| Last update date |
Oct 30, 2018 |
| Contact name |
Liang Guo |
| Organization name |
University of Minnesota
|
| Street address |
2001 6th Street SE
|
| City |
minneapolis |
| State/province |
MINNESOTA |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (47)
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| Relations |
| BioProject |
PRJNA488004 |
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