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| Status |
Public on Jul 17, 2019 |
| Title |
Ptpn2 regulates the generation of cytotoxic Tim-3+ CD8+ T cells and restrains anti-tumor immunity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Cellular and transcriptional experiments demonstrate that deletion of Ptpn2 enhances CD8+ T cell responses to chronic viral infection and cancer.
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| |
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| Overall design |
Murine bone-marrow chimeras were generated by intravenous transfer of lineage- Sca-1+ Kit+ (LSK) cells, derived from a CD45.1+ Cas9+ expressing mouse and infected with either control or Ptpn2-targeting sgRNAs, into irradiated recipient CD45.2 mice. CD8+ cells derived from the reconstituted hematopoetic compartment of the recipient mice were then isolated for transfer to host mice. For LCMV co-transfer studies, cells were transferred (500:500 mix) to recipient mice on day -1, and mice were infected with LCMV Clone 13 on day 0. For tumor co-transfer studies, cells were transferred (1000:1000 mix) to recipient mice on day -1, and mice were injected with MC38-OVA or B16-OVA on day 0. Day 7 or 8 post tumor or virus injection respectively, co-transferred T cells were isolated from the tumor or spleen (LCMV) (as above) and replicates of 500 cells were FACS sorted for transcriptional profiling.
A file with detailed methods is included at the foot of this record.
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| |
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| Contributor(s) |
LaFleur MW, Yates KB |
| Citation(s) |
31527834 |
| |
| Submission date |
Aug 30, 2018 |
| Last update date |
Oct 11, 2019 |
| Contact name |
Brian Christopher Miller |
| E-mail(s) |
Brianc_Miller@DFCI.HARVARD.EDU
|
| Organization name |
Harvard Medical School
|
| Department |
Immunology
|
| Lab |
Sharpe Lab
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (39)
|
| GSM3362909 |
Control-1 sgRNA Sample 1 Rep 1 [T19a8SP_r1_F11] |
| GSM3362910 |
Control-1 sgRNA Sample 1 Rep 2 [T19a8SP_r2_F12] |
| GSM3362911 |
Ptpn2-2 sgRNA Sample 1 Rep 1 [T20DP_r1_C11] |
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| This SubSeries is part of SuperSeries: |
| GSE134413 |
PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity |
|
| Relations |
| BioProject |
PRJNA488638 |
| SRA |
SRP159185 |
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