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| Status |
Public on Sep 01, 2018 |
| Title |
Tip60 complex promotes expression of a differentiation factor to regulate germline differentiation in female Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Germline stem cells (GSCs) self-renew and differentiate to sustain a continuous production of gametes. In the female Drosophila germ line, two differentiation factors, bag of marbles (bam) and benign gonial cell neoplasm (bgcn), work in concert in the stem cell daughter to promote the generation of eggs. In GSCs, bam transcription is repressed by signaling from the niche and is activated in stem cell daughters. In contrast, bgcn is transcribed in both the GSCs and stem cell daughters, but little is known about how bgcn is transcriptionally modulated. Here, we find that the conserved protein Nipped_A acts through the Tat interactive protein 60kDa (Tip60) Histone acetyl transferase (HAT) complex in the germ line to promote GSC differentiation. We find that Nipped_A is required for efficient exit from the gap phase 2 (G2) of cell cycle of the GSC daughter and for expression of differentiation factor bgcn. Loss of Nipped_A results in accumulation of GSC daughters. Forced expression of bgcn, in Nipped_A germline-depleted ovaries rescues this differentiation defect. Together, our results indicate that Tip60 complex coordinates cell cycle progression and expression of bgcn to help drive GSC daughters toward a differentiation program.
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| Overall design |
Two genotypes, 2 replicates each
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| Contributor(s) |
McCarthy A, Deiulio A, Martin ET, Upadhyay M, Rangan P |
| Citation(s) |
30230973 |
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| Submission date |
Aug 31, 2018 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Prashanth Rangan |
| E-mail(s) |
ranganlabualbany@gmail.com
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| Phone |
5184423485
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| Organization name |
RNA Institute
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| Department |
Biological Sciences
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| Lab |
Rangan Lab
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| Street address |
1400 Washington
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| City |
Albany |
| State/province |
NY |
| ZIP/Postal code |
12222 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA488790 |
| SRA |
SRP159253 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE119328_RAW.tar |
280.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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