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Status |
Public on Aug 31, 2019 |
Title |
DUX4-induced histone variants H3.X and H3.Y mark DUX4 target genes for expression (CUT&RUN) |
Organism |
Homo sapiens |
Experiment type |
Other
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Summary |
We determined the effects of short pulses of DUX4 expression on genome-wide transcription, mimicking the embryonic expression of this gene, using a DUX4-inducible cell culture model of FSHD (MB135iDUX4). We found that a second pulse of DUX4 has a synergistic effect and shows greater induction of DUX4 targets. Knockdown of DUX4-induced histone variants H3.X and H3.Y has no effect on DUX4 targets with a single pulse of DUX4 but eliminates the superinduction seen with a second pulse, showing that these histones are required for this increase in expression.
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Overall design |
genome-wide incorporation of histone variants H3.X and H3.Y in MB135iDUX4 myoblasts with overnight DUX4 induction, in triplicate, as well as IgG control
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Contributor(s) |
Resnick R, Wong C, Skene P, Hake S, Henikoff S, van der Maarel S, Tapscott S |
Citation(s) |
31722199 |
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Submission date |
Sep 04, 2018 |
Last update date |
Dec 05, 2019 |
Contact name |
Stephen Tapscott |
E-mail(s) |
stapscot@fredhutch.org
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Organization name |
Fred Hutch Cancer Research Center
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Department |
Human Biology
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Lab |
Tapscott
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Street address |
1100 Fairview N. Ave
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City |
Seattle |
State/province |
WASHINGTON |
ZIP/Postal code |
98103 |
Country |
USA |
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Platforms (1) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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Samples (8)
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This SubSeries is part of SuperSeries: |
GSE119403 |
DUX4-induced histone variants H3.X and H3.Y mark DUX4 target genes for expression |
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Relations |
BioProject |
PRJNA489182 |
SRA |
SRP159496 |