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Series GSE119401 Query DataSets for GSE119401
Status Public on Aug 31, 2019
Title DUX4-induced histone variants H3.X and H3.Y mark DUX4 target genes for expression (CUT&RUN)
Organism Homo sapiens
Experiment type Other
Summary We determined the effects of short pulses of DUX4 expression on genome-wide transcription, mimicking the embryonic expression of this gene, using a DUX4-inducible cell culture model of FSHD (MB135iDUX4). We found that a second pulse of DUX4 has a synergistic effect and shows greater induction of DUX4 targets. Knockdown of DUX4-induced histone variants H3.X and H3.Y has no effect on DUX4 targets with a single pulse of DUX4 but eliminates the superinduction seen with a second pulse, showing that these histones are required for this increase in expression.
Overall design genome-wide incorporation of histone variants H3.X and H3.Y in MB135iDUX4 myoblasts with overnight DUX4 induction, in triplicate, as well as IgG control
Contributor(s) Resnick R, Wong C, Skene P, Hake S, Henikoff S, van der Maarel S, Tapscott S
Citation(s) 31722199
Submission date Sep 04, 2018
Last update date Dec 05, 2019
Contact name Stephen Tapscott
Organization name Fred Hutch Cancer Research Center
Department Human Biology
Lab Tapscott
Street address 1100 Fairview N. Ave
City Seattle
State/province WASHINGTON
ZIP/Postal code 98103
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (8)
GSM3374168 Sample_RR_HsDm_1_DUX+
GSM3374169 Sample_RR_HsDm_2_DUX+
GSM3374170 Sample_RR_HsDm_3_DUX+
This SubSeries is part of SuperSeries:
GSE119403 DUX4-induced histone variants H3.X and H3.Y mark DUX4 target genes for expression
BioProject PRJNA489182
SRA SRP159496

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Supplementary file Size Download File type/resource
GSE119401_RAW.tar 1.2 Gb (http)(custom) TAR (of BW)
GSE119401_XYPlusVsIgG.bed.gz 423.2 Kb (ftp)(http) BED
GSE119401_XYPlusVsIgG.rda.gz 762.9 Kb (ftp)(http) RDA
GSE119401_XYPlusVsMinus.bed.gz 127.5 Kb (ftp)(http) BED
GSE119401_XYPlusVsMinus.rda.gz 325.2 Kb (ftp)(http) RDA
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Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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