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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 25, 2021 |
| Title |
Ectopic expression of Snord115 in choroid plexus interferes with editing but not splicing of 5-Ht2c receptor pre-mRNA in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Serotonin 5-HT2C receptor is a G-protein coupled excitatory receptor that regulates several biochemical pathways and has been implicated in obesity, mental state, sleep cycles, autism, neuropsychiatric disorders and neurodegenerative diseases. The activity of 5-HT2CR is regulated via alternative splicing and A to I editing of exon Vb of its pre-mRNA. Snord115 is a small nucleolar RNA that is expressed in mouse neurons and displays an 18-nucleotide base complementary to exon Vb of 5-HT2CR pre-mRNA. For almost two decades this putative guide element of Snord115 has wandered like a ghost through the literature in attempts to elucidate the biological significance of this complementarity. In mice, Snord115 is expressed in neurons and absent in the choroid plexus where, in contrast, 5-Ht2cr mRNA is highly abundant. Here we report the analysis of 5-Ht2cr pre-mRNA posttranscriptional processing via RNA deep sequencing in a mouse model that ectopically expresses Snord115 in the choroid plexus. In contrast to previous reports, our analysis demonstrated that Snord115 does not control alternative splicing of 5-Ht2cr pre-mRNA in vivo. We identified a modest, yet statistically significant reduction of 5-Ht2cr pre-mRNA A to I editing at the major A, B, C and D sites. We suggest that Snord115 and exon Vb of 5Ht2cr pre-mRNA form a double-stranded structure that is subject to ADAR-mediated A to I editing. To the best of our knowledge, this is the first comprehensive Snord115 gain-of-function analysis based on in vivo mouse models.
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| Overall design |
Comparison of two mouse models by RNA deep sequencing
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| Web link |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6414643/
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| Contributor(s) |
Raabe CA, Voss R, Kummerfeld D, Brosius J, Galiveti CR, Wolters A, Seggewiss J, Huge A, Skryabin BV, Rozhdestvensky TS |
| Citation(s) |
30862860 |
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| Submission date |
Sep 04, 2018 |
| Last update date |
Aug 26, 2021 |
| Contact name |
Timofey S. Rozhdestvensky |
| E-mail(s) |
rozhdest@uni-muenster.de
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| Organization name |
Medical Faculty, Core Facility Transgenic animal and genetic engineering Models (TRAM)
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| Department |
University of Muenster
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| Street address |
Von-Esmarch-Str. 56
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| City |
Muenster |
| State/province |
NRW |
| ZIP/Postal code |
48149 |
| Country |
Germany |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA489222 |
| SRA |
SRP159505 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE119419_5-Ht2c_splice_variants.fasta.gz |
211 b |
(ftp)(http) |
FASTA |
| GSE119419_Isoform_epression.xlsx |
32.0 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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