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| Status |
Public on Apr 25, 2019 |
| Title |
Single-cell RNA-seq analysis of human tonsil CD4 T cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed single-cell RNA-seq on CD4 T cells isolated from the tonsils of one healthy donor. We used the 10x chromium technology.
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| Overall design |
Tonsil CD4 T cells were enriched by negative selection using magnetic beads. Cell populations (CXCR5+PD-1low T cells, CXCR5+PD-1int T cells and CXCR5+PD-1high T cells ) were further isolated by cell sorting. Cellular suspensions (3500 cells) were loaded on a 10X Chromium instrument (10X Genomics) according to manufacturer’s protocol.
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| Contributor(s) |
Segura E, Durand M, Bohec M, Gueguen P |
| Citation(s) |
31072818 |
| |
| Submission date |
Sep 05, 2018 |
| Last update date |
Jul 25, 2019 |
| Contact name |
Paul Gueguen |
| E-mail(s) |
paul.gueguen@unil.ch
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| Organization name |
Institut Curie
|
| Department |
Inserm U932
|
| Street address |
26 rue D'Ulm
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA489436 |
| SRA |
SRP159651 |
