|
| Status |
Public on Oct 13, 2018 |
| Title |
Advantages of single nucleus over single cell RNA-seq in adult kidney |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A key limitation in single cell genomics is generating a high-quality single cell suspension that contains rare or difficult to dissociate cell types and is free of RNA degradation or transcriptional stress responses. Samples with unpredictable availability or that must be collected at several timepoints present additional challenges. Using adult mouse kidney, we compared single-cell RNA sequencing (scRNA-seq) data generated using DropSeq with snRNA-seq data generated from nuclei using sNuc-DropSeq, DroNc-seq and 10X Chromium. We validated snRNA-seq on fibrotic kidney from day 14 unilateral ureteral obstruction (UUO).
|
| |
|
| Overall design |
Dropseq, sNucDropseq, DroNcSeq and 10X Chromium were used to profile mouse healthy and fibrotic kidneys
|
| |
|
| Contributor(s) |
Humphreys BD |
| Citation(s) |
30510133 |
| |
| Submission date |
Sep 05, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Haojia Wu |
| E-mail(s) |
haojiawu@wustl.edu
|
| Organization name |
Washington University in St. Louis
|
| Department |
Renal Division/Internal Medicine
|
| Lab |
Humphreys Lab
|
| Street address |
600 S Euclid Ave
|
| City |
St. Louis |
| State/province |
Missouri |
| ZIP/Postal code |
MO 63110 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA489475 |
| SRA |
SRP159674 |
Less...
More...