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| Status |
Public on Sep 01, 2019 |
| Title |
Transcriptional profile of Mycobacterium tuberculosis mycP1-eccE1 deletion mutant |
| Organism |
Mycobacterium tuberculosis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mycobacterium tuberculosis is a slow-growing intracellular bacterium with the ability to induce host cell death and persist indefinitely in the human body. This pathogen uses the specialised ESX-1 secretion system to secrete virulence factors and potent immunogenic effectors required for disease progression. ESX-1 is a multi-subunit apparatus with a membrane complex that is predicted to form a pore in the cytoplasmic membrane. In M. tuberculosis this complex is made of five membrane proteins: EccB1, EccCa1, EccCb1, EccD1, EccE1. In this study, we have characterised the membrane component EccE1, and we found that deletion of eccE1 leads to disruption of ESX-1 secretion and attenuation of M. tuberculosis ex vivo.
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| Overall design |
In this study, we have characterized the membrane component EccE1, and we found that deletion of eccE1 leads to disruption of ESX-1 secretion and attenuation of M. tuberculosis ex vivo.
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| Contributor(s) |
Benjak A, Soler P, Cole ST |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Sep 06, 2018 |
| Last update date |
Sep 01, 2019 |
| Contact name |
Andrej Benjak |
| E-mail(s) |
andrej.benjak@unibe.ch
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| Organization name |
University of Bern
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| Department |
Department for BioMedical Research
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| Street address |
Murtenstrasse 24-28
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| City |
Bern |
| ZIP/Postal code |
3008 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL17879 |
Illumina HiSeq 2500 (Mycobacterium tuberculosis) |
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| Samples (12)
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| GSM3377905 |
M. tuberculosis H37Rv, control, replicate 1 |
| GSM3377906 |
M. tuberculosis H37Rv, control, replicate 2 |
| GSM3377907 |
M. tuberculosis H37Rv, delta mycP1-eccE1, replicate 1 |
| GSM3377908 |
M. tuberculosis H37Rv, delta mycP1-eccE1, replicate 2 |
| GSM3377909 |
M. tuberculosis H37Rv, delta mycP1-eccE1, complemented with mycP1-HA, replicate 1 |
| GSM3377910 |
M. tuberculosis H37Rv, delta mycP1-eccE1, complemented with mycP1-HA, replicate 2 |
| GSM3377911 |
M. tuberculosis H37Rv, delta mycP1-eccE1, complemented with eccE1-HA, replicate 1 |
| GSM3377912 |
M. tuberculosis H37Rv, delta mycP1-eccE1, complemented with eccE1-HA, replicate 2 |
| GSM3377913 |
M. tuberculosis H37Rv, delta mycP1-eccE1, complemented with mycP1-eccE1, replicate 1 |
| GSM3377914 |
M. tuberculosis H37Rv, delta mycP1-eccE1, complemented with mycP1-eccE1, replicate 2 |
| GSM3377915 |
M. tuberculosis H37Rv, delta mycP1-eccE1, with empty vector, replicate 1 |
| GSM3377916 |
M. tuberculosis H37Rv, delta mycP1-eccE1, with empty vector, replicate 2 |
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| Relations |
| BioProject |
PRJNA489657 |
| SRA |
SRP159843 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE119582_CountTable_no-rRNA.txt.gz |
102.0 Kb |
(ftp)(http) |
TXT |
| GSE119582_DESeq2_resLFC_delta-mycP1-eccE1_complemented-with-eccE1_vs_Rv-wt.csv.gz |
220.3 Kb |
(ftp)(http) |
CSV |
| GSE119582_DESeq2_resLFC_delta-mycP1-eccE1_complemented-with-mycP1-eccE1_vs_Rv-wt.csv.gz |
215.5 Kb |
(ftp)(http) |
CSV |
| GSE119582_DESeq2_resLFC_delta-mycP1-eccE1_complemented-with-mycP1_vs_Rv-wt.csv.gz |
221.8 Kb |
(ftp)(http) |
CSV |
| GSE119582_DESeq2_resLFC_delta-mycP1-eccE1_vs_Rv-wt.csv.gz |
205.3 Kb |
(ftp)(http) |
CSV |
| GSE119582_DESeq2_resLFC_delta-mycP1-eccE1_with-EmptyVector_vs_Rv-wt.csv.gz |
212.7 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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