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Series GSE119787 Query DataSets for GSE119787
Status Public on Sep 12, 2018
Title Rec114 ChIP-seq
Organisms Saccharomyces cerevisiae; Saccharomyces mikatae
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary To segregate accurately during meiosis, homologous chromosomes in most species must recombine. Very small chromosomes would risk missegregation if recombination were randomly distributed, so the double-strand breaks (DSBs) that initiate recombination are not haphazard. How this nonrandomness is controlled is not understood. Here we demonstrate that Saccharomyces cerevisiae integrates multiple, temporally distinct pathways to regulate chromosomal binding of pro-DSB factors Rec114 and Mer2, thereby controlling duration of a DSB-competent state. Homologous chromosome engagement regulates Rec114/Mer2 dissociation late in prophase, whereas replication timing and proximity to centromeres or telomeres influence timing and amount of Rec114/Mer2 accumulation early. A distinct early mechanism boosts Rec114/Mer2 binding quickly to high levels specifically on the shortest chromosomes, dependent on chromosome axis proteins and subject to selection pressure to maintain hyperrecombinogenic properties of these chromosomes. Thus, an organism’s karyotype and its attendant risk of meiotic missegregation influence the shape and evolution of its recombination landscape.
 
Overall design Sixty samples total: 3 time points (each time points contains ChIP and input samples) from Rec114-myc wild type, zip3, hop1, red1, rec8, hop1 red1, hop1 red1 rec8 strains, strains with reciprocal translocation (between chr1 and chr4, or chr8 and chr9) in S. cerevisiae, and Rec114-myc wild type in S. mikatae.
 
Contributor(s) Murakami H, Keeney S
Citation(s) 32494071
Submission date Sep 11, 2018
Last update date Jun 11, 2020
Contact name Hajime Murakami
E-mail(s) murakamh@mskcc.org
Phone 2126395180
Organization name Memorial Sloan Kettering Cancer Center
Department Molecular Biology
Lab Keeney
Street address 1275 york ave
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platforms (2)
GPL17342 Illumina HiSeq 2500 (Saccharomyces cerevisiae)
GPL20788 Illumina HiSeq 2500 (Saccharomyces mikatae)
Samples (60)
GSM3383780 wild type ChIP 2h
GSM3383781 wild type ChIP 4h
GSM3383782 wild type ChIP 6h
Relations
BioProject PRJNA490297
SRA SRP161477

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE119787_processed_data_files.tar.gz 482.9 Mb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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