|
| Status |
Public on Sep 01, 2019 |
| Title |
Morphological and transcriptomic analysis reveal robust nonhost resistance response during chickpea interaction with Alternaria brassicae |
| Organism |
Cicer arietinum |
| Experiment type |
Expression profiling by array
|
| Summary |
Alternaria brassicae is a necrotrophic fungal pathogen which infects brassica crops and lead to huge loss in crop production. There is a need to exploit the novel resistance mechanism against A. brassicae. Under field condition, chickpea is the potential nonhost plant for A. brassicae. At molecular level, it is not known how the NHR in chickpea operates against A. brassicae. In present study, we did the transcriptomic analysis in chickpea plants exposed to nonhost pathogen, A. brassicae by using microarray. Chickpea plants were spray inoculated with the spore suspension of A. brassicae. The leaf samples were harvested after 24 hpi and 48 hpi from pathogen treated plants and from the mock-treated control plants. The Transcriptome analysis were done from the leaf samples obtained at both the time-points by microarray using Agilent ChickpeaGXP_8X60K chip. Our result suggested the robust transcriptional reprogramming leading to defense response against A. brassicae.
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| Overall design |
Organism : Chickpea, Agilent custom Chickpea Gene Expression 8x60k Array (AMADID: 037094) designed by Genotypic Technology Private Limited
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| Contributor(s) |
Fatima U, Bhorali P, Senthil-Kumar M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 12, 2018 |
| Last update date |
Sep 03, 2019 |
| Contact name |
Senthil-Kumar Muthappa |
| E-mail(s) |
skmuthappa@nipgr.ac.in
|
| Phone |
+91-8826140777
|
| Organization name |
National Institute of Plant Genome Research
|
| Lab |
107
|
| Street address |
Aruna Asaf Ali Road
|
| City |
New Delhi |
| ZIP/Postal code |
110067 |
| Country |
India |
| |
|
| Platforms (1) |
| GPL21347 |
Custom Agilent Chickpea Whole Genome 8x60K (AMADID: 037094) designed by Genotypic Technology Private Limited |
|
| Samples (8)
|
| GSM3386114 |
C1: leaf water treated as mock control, biological rep1 |
| GSM3386115 |
C2: leaf water treated as mock control, biological rep2 |
| GSM3386116 |
C3: leaf water treated as mock control, biological rep3 |
| GSM3386117 |
T1: Leaf Alternaria brassicae treated after 24 hpi, biological rep1 |
| GSM3386118 |
T2: Leaf Alternaria brassicae treated after 24 hpi, biological rep2 |
| GSM3386119 |
T3: Leaf Alternaria brassicae treated after 24 hpi, biological rep3 |
| GSM3386120 |
T4: Leaf Alternaria brassicae treated after 48 hpi, biological rep1 |
| GSM3386121 |
T5: Leaf Alternaria brassicae treated after 48 hpi, biological rep2 |
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| Relations |
| BioProject |
PRJNA490503 |
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