GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE119941 Query DataSets for GSE119941
Status Public on Apr 16, 2019
Title Single-Cell Transcriptomics Identifies TOX as a Key Transcriptional Regulator of Progenitor-Like CD8 T Cells in Chronic Infection [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Stem-like CD8 T cells maintain long-term antiviral CD8 immunity during chronic infection, and share regulatory pathways with memory precursor effector cells generated after acute infection. However, it is unclear whether stem-like CD8 T cells require distinct transcriptional and epigenetic regulation for their longevity and adaptation to the immunosuppressive environment in chronic infection. Here, our comparison of single-cell transcriptomes and epigenetic profiles of CD8 T cells responding to acute and chronic viral infections revealed that stem-like CD8 T cells became distinct from memory precursors before clonal expansion ended. We found that a coexpression gene module containing Tox exhibited higher transcriptional activities and active histone marks in stem-like T cells than memory precursors. Moreover, TOX promoted persistence of antiviral CD8 T cells, and was required for stem-like CD8 differentiation. Our results indicate that stem-like CD8 T cells require unique transcriptional and epigenetic programs for their differentiation and persistence during chronic viral infection.
Overall design Single-cell tcranscriptomic and epigentic analysis of antigen specific CD8 T cells after acute and chronic viral infeciton. Transcrptome analysis of TOX over expression in antigen specific CD8 T cells after chornic viral infection.
Contributor(s) Yao C, Sun HW, O'Shea JJ, Schwartzberg PL, Wu TQ
Citation(s) 31209400
Submission date Sep 13, 2018
Last update date Jul 03, 2019
Contact name Tuoqi Wu
Organization name National Institute of Allergy and Infectious Diseases
Street address 49 Convent Dr.
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (16)
GSM3388379 Chipseq_H3K27Ac_P14_Arm_D7_ShortLivedEffector_1
GSM3388380 Chipseq_H3K27Ac_P14_Arm_D7_ShortLivedEffector_2
GSM3388381 Chipseq_H3K27Ac_P14_Arm_D7_MemoryPrecursor_1
This SubSeries is part of SuperSeries:
GSE119943 Single-Cell Transcriptomics Identifies TOX as a Key Transcriptional Regulator of Progenitor-Like CD8 T Cells in Chronic Infection
BioProject PRJNA490749
SRA SRP161708

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE119941_RAW.tar 327.1 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap