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| Status |
Public on Feb 11, 2019 |
| Title |
PfAP2-G ChIP-seq in committed schizonts, sexual rings, and stage I gametocytes |
| Organism |
Plasmodium falciparum |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In the malaria parasite Plasmodium falciparum, the switch from asexual multiplication to sexual differentiation into gametocytes is essential for transmission to mosquitos. One of the key determinants of sexual commitment is the transcription factor PfAP2-G, which has been proposed to orchestrate this crucial cell fate decision by driving expression of gametocyte genes. We show conclusively that PfAP2-G is a transcriptional activator of gametocyte genes and identify the earliest known markers expressed during commitment. Remarkably, we also find that in sexually committed cells, PfAP2-G is associated with the promoters of genes important for red blood cell invasion and activates them through its interactions with a second transcription factor. We thus demonstrate an intriguing transcriptional link between the apparently opposing processes of red blood cell invasion and gametocytogenesis that is coordinated by the master regulator PfAP2-G. This finding has important implications for the development of new anti-malarial drugs that block the invasion of red blood cells by sexually committed cells, thereby preventing parasite transmission.
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| Overall design |
ChIP was performed on the AP2-G-DD line (+Shld1) at schizont, ring, and stage I gametocyte stages using an antibody against HA. There are two biological replicates for each of the three stages, giving a total of six samples. The input material served as the control.
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| Contributor(s) |
Josling GA, Venezia J, Orchard L, Russell TJ, Painter H, Llinás M |
| Citation(s) |
32198457 |
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| Submission date |
Sep 25, 2018 |
| Last update date |
Mar 30, 2020 |
| Contact name |
Manuel Llinas |
| E-mail(s) |
manuel@psu.edu
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| Organization name |
Pennsylvania State University
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| Department |
Biochemistry and Molecular Biology
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| Street address |
Millennium Science Complex
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| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
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| Platforms (1) |
| GPL21078 |
Illumina HiSeq 2500 (Plasmodium falciparum) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE125566 |
Regulation of sexual differentiation is linked to invasion in malaria parasites |
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| Relations |
| BioProject |
PRJNA493084 |
| SRA |
SRP162601 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE120448_RAW.tar |
138.4 Mb |
(http)(custom) |
TAR (of BED, BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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