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| Status |
Public on May 05, 2020 |
| Title |
The AML-associated K313 mutation enhances C/EBPα activity by increasing translation via release of ribosome stalling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Mutations in the transcription factor C/EBPα are found in about 10% of all acute myeloid leukaemia (AML) cases but the contribution of these mutations to leukaemogenesis is incompletely understood. We here use a mouse model of granulocyte progenitors expressing conditionally active HoxB8 to assess the cell biological and molecular activity of C/EBPα-mutations associated with human AML.
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| Overall design |
Total RNA was isolated from HoxB8 neutrophil proginitor cells expressing WT or K313-C/EBPα (day 1 of differentiation). Triplicates were used for hybridization on the Affymetrix Mouse Clariom™ S microarray.
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| Contributor(s) |
Moelter I, Schott J, Lindner D, Stoecklin G, Döhner K, Lübbert M, Badr MT, Gentle IE, Häcker G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 25, 2018 |
| Last update date |
May 05, 2020 |
| Contact name |
Mohamed Tarek Badr |
| E-mail(s) |
tarekzakaria.badr@gmail.com
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| Organization name |
University of Freiburg
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| Department |
Institute of Medical Microbiology and Hygiene
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| Street address |
Hermann-Herder-Str. 11
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| City |
Freiburg |
| ZIP/Postal code |
79104 |
| Country |
Germany |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (6)
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| GSM3401246 |
neutrophil progenitor cells expressing WT-C/EBPα rep1 |
| GSM3401247 |
neutrophil progenitor cells expressing WT-C/EBPα rep2 |
| GSM3401248 |
neutrophil progenitor cells expressing WT-C/EBPα rep3 |
| GSM3401249 |
neutrophil progenitor cells expressing K313-C/EBPα rep1 |
| GSM3401250 |
neutrophil progenitor cells expressing K313-C/EBPα rep2 |
| GSM3401251 |
neutrophil progenitor cells expressing K313-C/EBPα rep3 |
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| Relations |
| BioProject |
PRJNA493112 |
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