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Series GSE120544 Query DataSets for GSE120544
Status Public on Sep 27, 2018
Title Butyrivibrio hungatei MB2003 and Butyrivibrio proteoclasticus B316T grown in mono- and co-cultures on xylan or pectin
Organisms Butyrivibrio proteoclasticus; Butyrivibrio hungatei
Experiment type Expression profiling by high throughput sequencing
Summary Rumen bacterial species belonging to the genera Butyrivibrio are important degraders of plant polysaccharides, particularly hemicelluloses (arabinoxylans) and pectin. Currently, four distinct species are recognized which have very similar substrate utilization profiles, but little is known about how these microorganisms are able to co-exist in the rumen. To investigate this question, Butyrivibrio hungatei MB2003 and Butyrivibrio proteoclasticus B316T were grown alone or in co-culture on the insoluble substrates, xylan or pectin, and their growth, release of sugars, fermentation end products and transcriptomes were examined. In single cultures, B316T was able to degrade and grow well on xylan and pectin, while MB2003 was unable to utilize either of these insoluble substrates to support significant growth. Co-cultures of B316T grown with MB2003 revealed that MB2003 showed almost equivalent growth to B316T when either xylan or pectin were supplied as substrates. The effect of co-culture on the transcriptomes of B316T and MB2003 was very marked; B316T transcription was largely unaffected by the presence MB2003, but MB2003 expressed a wide range of genes encoding carbohydrate degradation/metabolism and oligosaccharide transport/assimilation in order to compete with B316T for the released sugars. These results suggest that B316T has a role as an initiator of the primary solubilization of xylan and pectin, while MB2003 competes effectively as a scavenger for the released soluble sugars to enable its growth and maintenance in the rumen.
 
Overall design Transcriptional profiling of B. hungatei MB2003 and B. proteoclasticus B316T grown in mono- and co-cultures on xylan and pectin substrates in triplicate.
 
Contributor(s) Palevich N, Attwood GT, Kelly WJ, Rakonjac J
Citation(s) 30478228
Submission date Sep 26, 2018
Last update date Jan 06, 2019
Contact name Nikola Palevich
E-mail(s) nik.palevich@agresearch.co.nz
Phone 021972511
Organization name AgResearch
Street address Tennent Drive, Agresearch, Grasslands
City Palmerston North
State/province Manawatu
ZIP/Postal code 4410
Country New Zealand
 
Platforms (3)
GPL25614 Illumina HiSeq 2000 (Butyrivibrio hungatei)
GPL25615 Illumina HiSeq 2000 (Butyrivibrio proteoclasticus)
GPL25616 Illumina HiSeq 2000 (Butyrivibrio hungatei; Butyrivibrio proteoclasticus)
Samples (18)
GSM3402776 MB2003-1-Xylan
GSM3402777 MB2003-2-Xylan
GSM3402778 MB2003-3-Xylan
Relations
BioProject PRJNA493497
SRA SRP162706

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE120544_CP017830_Pectin_transcripts.txt.gz 10.8 Kb (ftp)(http) TXT
GSE120544_CP017830_Xylan_transcripts.txt.gz 9.1 Kb (ftp)(http) TXT
GSE120544_CP017831_Pectin_transcripts.txt.gz 239.5 Kb (ftp)(http) TXT
GSE120544_CP017831_Xylan_transcripts.txt.gz 244.9 Kb (ftp)(http) TXT
GSE120544_CP017832_Pectin_transcripts.txt.gz 14.5 Kb (ftp)(http) TXT
GSE120544_CP017832_Xylan_transcripts.txt.gz 13.6 Kb (ftp)(http) TXT
GSE120544_CP017833_Pectin_transcripts.txt.gz 679 b (ftp)(http) TXT
GSE120544_CP017833_Xylan_transcripts.txt.gz 676 b (ftp)(http) TXT
GSE120544_NC_014387_Pectin_transcripts.txt.gz 134.9 Kb (ftp)(http) TXT
GSE120544_NC_014387_Xylan_transcripts.txt.gz 249.2 Kb (ftp)(http) TXT
GSE120544_NC_014388_Pectin_transcripts.txt.gz 13.3 Kb (ftp)(http) TXT
GSE120544_NC_014388_Xylan_transcripts.txt.gz 19.0 Kb (ftp)(http) TXT
GSE120544_NC_014389_Pectin_transcripts.txt.gz 16.1 Kb (ftp)(http) TXT
GSE120544_NC_014389_Xylan_transcripts.txt.gz 25.0 Kb (ftp)(http) TXT
GSE120544_NC_014390_Pectin_transcripts.txt.gz 7.1 Kb (ftp)(http) TXT
GSE120544_NC_014390_Xylan_transcripts.txt.gz 10.5 Kb (ftp)(http) TXT
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