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| Status |
Public on Apr 08, 2020 |
| Title |
Tumor Necrosis Factor Alpha Regulates Skeletal Myogenesis by Inhibiting SP1 Interaction with cis-Acting Regulatory Elements within the Fbxl2 Gene Promoter |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using NGS technology we describe the transcriptomic effecs of TNFα stimulation on skeletal myogenesis and, through gene sliencing techniques, compare the effects of TNFα with the loss of a critical cell cycle regulator, Fbxl2, on the process of myogenic proliferation and differentiation.
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| Overall design |
RNA sequencing was performed on C2C12 cells during the stage of myoblast proliferation (proliferation), at 48 h post induction of differentiation (control), in the presence of 10 ng/ml TNFα stimulation (TNF) at 48 h, and after Fbxl2 gene silencing at 48 h post induction of differentiation.
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| |
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| Contributor(s) |
O'Brien ME |
| Citation(s) |
32205409 |
| Submission date |
Sep 28, 2018 |
| Last update date |
Jul 08, 2020 |
| Contact name |
Michael Emmet O'Brien |
| Organization name |
University of Pittsburgh
|
| Department |
Pulmonary, Allergy, and Critical Care Medicine
|
| Lab |
Mallampalli
|
| Street address |
200 Lothrop Street
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (12)
|
| GSM3405592 |
Day0_A_S1_R1_001: Proliferation (Day 0) |
| GSM3405593 |
Day0_B_S10_R1_001: Proliferation (Day 0) |
| GSM3405594 |
Day0_C_S11_R1_001: Proliferation (Day 0) |
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| Relations |
| BioProject |
PRJNA493731 |
| SRA |
SRP162815 |
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