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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 26, 2018 |
| Title |
TL1A is a central regulator of group 3 innate lymphoid cell function in colitis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Inflammatory bowel disease (IBD) results from a dysregulated interaction between the microbiota and a genetically susceptible host. Genetic studies have linked TNFSF15 polymorphisms and its protein TNF-like ligand 1A (TL1A) with IBD, but the functional role of TL1A in linking tissue homeostasis and intestinal inflammation is not known. Here, using cell-specific genetic deletion models, we report an essential role for CX3CR1+ mononuclear phagocyte (MNP)-derived TL1A, which is induced by adherent IBD-associated microbiota, in regulating group 3 innate lymphoid cell (ILC3) production of IL-22 and mucosal healing in acute colitis. However, in contrast to this protective role in acute colitis, TL1A-dependent expression of OX40L in MHCII+ ILC3 during colitis leads to co-stimulation of antigen-specific T cells and is required for chronic T cell colitis. These results identify a new role for ILC3 in regulating intestinal T cells and reveal a central role for TL1A in regulating ILC3 barrier immunity during colitis.
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| Overall design |
RNA from media- or TL1A-stimulated sorted Lin-CD127+IL23R-GFP+ ILC3s from IL23R-GFP/WT mice
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| Contributor(s) |
Putzel GG, Longman RS |
| Citation(s) |
30552020 |
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| Submission date |
Oct 01, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Gregory Putzel |
| Organization name |
NYU Langone Health
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| Department |
Microbiology
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| Lab |
Pironti
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| Street address |
430 East 29th Street
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA494250 |
| SRA |
SRP163086 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE120723_gene_counts.tsv.gz |
185.8 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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