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Series GSE120726 Query DataSets for GSE120726
Status Public on Oct 03, 2018
Title The small protein MgtS and small RNA MgrR modulate the PitA phosphate symporter to boost intracellular magnesium levels
Platform organism Escherichia coli
Sample organism Escherichia coli str. K-12 substr. MG1655
Experiment type Expression profiling by array
Summary In response to low levels of magnesium (Mg2+), the PhoQP two component system induces the transcription of two convergent genes, one encoding a 31-amino acid protein denoted MgtS and the second encoding a small, regulatory RNA (sRNA) denoted MgrR. Previous studies showed that the MgtS protein interacts with and stabilizes the MgtA Mg2+ importer to increase intracellular Mg2+ levels, while the MgrR sRNA base pairs with the eptB mRNA thus affecting lipopolysaccharide modification. Surprisingly, we found overexpression of the MgtS protein also leads to induction of the PhoRB regulon. Studies to understand this activation showed that MgtS forms a complex with a second protein, PitA, a cation-phosphate symporter. Given that the additive effect of ∆mgtA and ∆mgtS mutations on intracellular Mg2+ concentrations seen previously is lost in the ∆pitA mutant, we suggest that MgtS binds to and blocks Mg2+ leakage through PitA under Mg2+-limiting conditions. Consistent with a detrimental role of PitA in low Mg2+, we also observe MgrR sRNA repression of PitA synthesis. Thus, PhoQP induces the expression of two convergent small genes in response to Mg2+ limitation whose products both repress PitA activity and levels to increase intracellular Mg2+ levels.

Funded by NIH Intramural Program ZIA HD008855-11.
 
Overall design Two different strains: one overexpressing mgtS (yneM) and one containing empty vector.
 
Contributor(s) Xuefeng Y, Mona O, Hanbo W, Errett H, Svetlana S, Gisela S
Citation(s) 30276893
Submission date Oct 02, 2018
Last update date Mar 08, 2019
Contact name Gisela Storz
E-mail(s) storzg@mail.nih.gov
Phone 301-402-0968
Organization name NICHD
Department Division of Molecular and Cellular Biology
Street address 49 Convent Drive
City Bethesda
State/province MD
ZIP/Postal code 20892-4417
Country USA
 
Platforms (1)
GPL3154 [E_coli_2] Affymetrix E. coli Genome 2.0 Array
Samples (4)
GSM3409404 ECH (20091218) Sample_2_empty_vector
GSM3409405 ECH (20091218) Sample_1_yneM_overexpressed
GSM3409406 XY (20110126) E_coli_2
Relations
BioProject PRJNA494307

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE120726_RAW.tar 9.2 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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