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| Status |
Public on Sep 30, 2019 |
| Title |
Transcriptional landscape of pulmonary lymphatic endothelium during late gestation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Maturation of lung cell types occurs during late gestation to ensure lung function and optimal gas exchange at birth. The pulmonary lymphatic endothelium is an understudied cell type and is essential for immune regulation and interstitial fluid removal. Here we provide the first characterization of the pulmonary lymphatic endothelium during late gestation prior to birth.
We used microarrays to detail the global transcriptional programme underlying maturation of pulmonary lymphatic endothelium and identified 1,281 genes with significant changes in gene expression over time. Gene expression was validated by qPCR and in situ hybridization, which indicated a potential role for IFN-I signaling in pulmonary lymphatic endothelial cell (PLEC) maturation prior to birth.
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| Overall design |
After microdissection in ice-cold PBS, we combined 10-21 embryonic lungs from two litters for each sample at E16.5, E17.5, and E18.5 fetal time points (n=3 samples/time point). After tissue digestion and immunostaining, we sorted CD45-/Epcam-/CD31+/Vegfr3+/Lyve1+/Pdpn+ cells from the fetal murine lungs directly into QIAzol on a FACS Aria SORP. RNA extraction was performed using the miRNeasy kit (Qiagen). RNA (RIN>7.5) was used to profile gene expression with Affymetrix Mouse Gene 2.0 microarrays in the Boston University Microarray and Sequencing Resource.
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| Contributor(s) |
Norman T, Fine A |
| Citation(s) |
31083674 |
| Submission date |
Oct 10, 2018 |
| Last update date |
Oct 02, 2019 |
| Contact name |
Boston University Microarray and Sequencing Resource |
| E-mail(s) |
msrdata@bu.edu
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| Organization name |
Boston University
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| Department |
Microarray and Sequencing Resource
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| Street address |
72 East Concord Street, E631
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
| GPL17791 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [mogene20st_Mm_ENTREZG_17.1.0] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA495591 |
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