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| Status |
Public on Dec 31, 2018 |
| Title |
Comparison of DNA methyltransferase-1 (Dnmt1) deleted urogenital sinus epithelium compared to wildtype urogenital sinus epithelium |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Dnmt1 is an important regulator of tissue development and differentiation. To assess the effects of epithelium Dnmt1 deletion in the developing urogenital sinus (precursor of the urethra and prostate in males), we isolated urogenital sinus epithelial tissue from Dnmt1 deleted mouse embryos and wildtype mouse embryos. The transcriptomes were analyzed by RNA-seq
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| Overall design |
A Shh cre driver was used to achieve Dnmt1 deletion in the epithelium of the developing urogenital sinus. Epithelium of the urogenital sinus of embryonic day 15.5 mouse embryos were isolated by trypsin digestion. Tissues from Dnmt1 mutants (Dnmt1cKO) were compared to wildtype embryos (Control) in this experiment.
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| Contributor(s) |
Joseph DB, Vezina CM, Chu L |
| Citation(s) |
30659795 |
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| Submission date |
Oct 10, 2018 |
| Last update date |
Apr 01, 2019 |
| Contact name |
Chad Michael Vezina |
| Organization name |
University of Wisconsin-Madison
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| Department |
Comparative Biosciences
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| Street address |
1656 Linden Drive
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| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA495601 |
| SRA |
SRP164916 |
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