|
| Status |
Public on Dec 10, 2018 |
| Title |
Urinary miRNAs contained in exosome of dogs and cats |
| Organisms |
Canis lupus familiaris; Felis catus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
The purpose of this study was the identification of RNAs contained in the urinary exosome (UExo) from dogs and cats. The quality of total RNA in isolated urinary exosome (UExo)-derived total RNAs obtained from the column-based method (urine 1 mL) was checked by using a Bioanalyzer, and samples from normal renal function (NR) group and kidney disease (KD) group were pooled as one sample for each group. We collected NR dogs (n = 37), KD dogs (n = 47), NR cats (n=43), and KD cats (n = 45). For the next generation sequencing, libraries were prepared according to the manufacturer’s protocols and sequenced using 50-base reads acquired by using a HiSeq 2000 platform. The December 2011 (GRCm38/mm10) mouse (Mus musculus) genome data were used as reference. As a result, we could identify the miRNA from these samples.
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| |
|
| Overall design |
Examination of NR dogs (n = 37), KD dogs (n = 47), NR cats (n=43), and KD cats (n = 45) which were pooled as one sample for each group.
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| |
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| Contributor(s) |
Ichii O |
| Citation(s) |
30525049 |
| |
| Submission date |
Oct 11, 2018 |
| Last update date |
Dec 26, 2018 |
| Contact name |
Osamu Ichii |
| E-mail(s) |
ichi-o@vetmed.hokudai.ac.jp
|
| Organization name |
Hokkaido University
|
| Department |
Faculty of Veterinary Medicine
|
| Lab |
Anatomy
|
| Street address |
Kita 18-Nishi 9
|
| City |
Sapporo |
| State/province |
Hokkaido |
| ZIP/Postal code |
060-0818 |
| Country |
Japan |
| |
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| Platforms (2) |
| GPL25667 |
Illumina HiSeq 1500 (Canis lupus familiaris) |
| GPL25668 |
Illumina HiSeq 1500 (Felis catus) |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA495859 |
| SRA |
SRP165238 |
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