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Series GSE121217 Query DataSets for GSE121217
Status Public on Oct 16, 2018
Title Global gene expression analysis in PTEN versus GFP expressing H1650 lung adenocarcinoma cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary PTEN or GFP were stably expressed in PTEN-deficient lung adenocarcinoma cells by viral transfection followed by puromycin selection. Microarray analysis of total RNA isolated from H1650 cells stably expressing PTEN or GFP was performed to identify potential PTEN responsive genes.
 
Overall design Three biological replicate retroviral infections of H1650 cells were performed with pBABE-PTEN or pBABE-GFP expression plasmidsollowed by selection in 2 ug/ml puromycin for 2 days. Stable PTEN expression was confirmed by immunoblot analysis 5 days post-infection. Total RNA was isolated from each replicate using the RNeasy kit (Qiagen) according to manufacturer’s instructions. After quality control validation, total RNA was processed for labeling and hybridization on GeneChip Human Genome Arrays (HGU133 plus 2.0) followed by scanning and image quantification.
 
Contributor(s) Bivona T
Citation(s) 31461649
Submission date Oct 15, 2018
Last update date Aug 31, 2021
Contact name Nilanjana Chatterjee
E-mail(s) nilanjana.chatterjee@ucsf.edu
Organization name UCSF
Department Department of Medicine
Lab Dr. Bivona
Street address 600 16th street
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM3428040 H1650_PTEN.deficient_cells 1
GSM3428041 H1650_PTEN.deficient_cells 2
GSM3428042 H1650_PTEN.deficient_cells 3
Relations
BioProject PRJNA496393

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE121217_RAW.tar 30.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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