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Status |
Public on Oct 16, 2018 |
Title |
Global gene expression analysis in PTEN versus GFP expressing H1650 lung adenocarcinoma cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
PTEN or GFP were stably expressed in PTEN-deficient lung adenocarcinoma cells by viral transfection followed by puromycin selection. Microarray analysis of total RNA isolated from H1650 cells stably expressing PTEN or GFP was performed to identify potential PTEN responsive genes.
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Overall design |
Three biological replicate retroviral infections of H1650 cells were performed with pBABE-PTEN or pBABE-GFP expression plasmidsollowed by selection in 2 ug/ml puromycin for 2 days. Stable PTEN expression was confirmed by immunoblot analysis 5 days post-infection. Total RNA was isolated from each replicate using the RNeasy kit (Qiagen) according to manufacturer’s instructions. After quality control validation, total RNA was processed for labeling and hybridization on GeneChip Human Genome Arrays (HGU133 plus 2.0) followed by scanning and image quantification.
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Contributor(s) |
Bivona T |
Citation(s) |
31461649 |
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Submission date |
Oct 15, 2018 |
Last update date |
Aug 31, 2021 |
Contact name |
Nilanjana Chatterjee |
E-mail(s) |
nilanjana.chatterjee@ucsf.edu
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Organization name |
UCSF
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Department |
Department of Medicine
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Lab |
Dr. Bivona
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Street address |
600 16th street
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City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94143 |
Country |
USA |
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Platforms (1) |
GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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Samples (6)
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Relations |
BioProject |
PRJNA496393 |