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Series GSE121333 Query DataSets for GSE121333
Status Public on Nov 30, 2018
Title Context dependent Histone H3 Lysine 4 methylation is necessary for repression and is a requisite modification for facultative heterochromatin at distinct loci [ChIP-seq]
Organism Neurospora crassa
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Sensing and responding to light provides organisms an adaptive advantage, in part by altering gene expression. The complement of light-activated genes in model organisms is largely known, and some of the mechanisms by which proteins modulate the light response are likewise well defined. However, how light alters post translation modifications to chromatin and how changes in chromatin facilitates and/or inhibit changes in gene expression has not been examined in depth. Nor is know how specific chromatin-modifiers assist in modulating the light response or the extent of the defects in strains lacking certain chromatin modifications. Using a combination of RNA-seq and ChIP-seq we examines H3K4 methylation and H3K9 methylation and found paradigm changing properties beyond the consensus that H3K4me3 is a solely a mark for activation and H3K9me3 marks silent heterochromatin domains.
 
Overall design Samples consist of different strains, harvest times, and antibodies.
 
Contributor(s) J.Belden W, Zhu Q, Ramakrishnan M, Park J
Citation(s) 31068130
Submission date Oct 16, 2018
Last update date May 21, 2019
Contact name William J. Belden
E-mail(s) beldenwj@scarletmail.rutgers.edu
Phone 848-932-5617
Organization name Rutgers, The State University of New Jersey
Department Animal Sciences
Lab Belden Lab
Street address 59 Dudley Rd
City New Brunswick
State/province NJ
ZIP/Postal code 08091
Country USA
 
Platforms (1)
GPL20705 Illumina HiSeq 2500 (Neurospora crassa)
Samples (20)
GSM3431715 WT_DD_K9_rep1
GSM3431716 WT_DD_K9_rep2
GSM3431717 WT_LP_K9_rep1
This SubSeries is part of SuperSeries:
GSE121356 Context dependent Histone H3 Lysine 4 methylation is necessary for repression and is a requisite modification for facultative heterochromatin at distinct loci
Relations
BioProject PRJNA497093
SRA SRP165901

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE121333_WT_DD_GSE121333_macs2_replicates_p_peaks.bed.gz 18.5 Kb (ftp)(http) BED
GSE121333_WT_LP30_GSE121333_macs2_replicates_p_peaks.bed.gz 18.8 Kb (ftp)(http) BED
GSE121333_macs2_K4_Dim5_DD_peaks.bed.gz 134.6 Kb (ftp)(http) BED
GSE121333_macs2_K4_GSE121333_WT_DD_peaks.bed.gz 124.4 Kb (ftp)(http) BED
GSE121333_macs2_K4_GSE121333_WT_LP30_peaks.bed.gz 127.2 Kb (ftp)(http) BED
GSE121333_macs2_K4_dim5_LP30_peaks.bed.gz 134.6 Kb (ftp)(http) BED
GSE121333_set1_DD_GSE121333_macs2_replicates_p_peaks.bed.gz 22.0 Kb (ftp)(http) BED
GSE121333_set1_LP30_GSE121333_macs2_replicates_p_peaks.bed.gz 20.8 Kb (ftp)(http) BED
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Raw data are available in SRA
Processed data are available on Series record

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