|
| Status |
Public on Nov 30, 2018 |
| Title |
Context dependent Histone H3 Lysine 4 methylation is necessary for repression and is a requisite modification for facultative heterochromatin at distinct loci [ChIP-seq] |
| Organism |
Neurospora crassa |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Sensing and responding to light provides organisms an adaptive advantage, in part by altering gene expression. The complement of light-activated genes in model organisms is largely known, and some of the mechanisms by which proteins modulate the light response are likewise well defined. However, how light alters post translation modifications to chromatin and how changes in chromatin facilitates and/or inhibit changes in gene expression has not been examined in depth. Nor is know how specific chromatin-modifiers assist in modulating the light response or the extent of the defects in strains lacking certain chromatin modifications. Using a combination of RNA-seq and ChIP-seq we examines H3K4 methylation and H3K9 methylation and found paradigm changing properties beyond the consensus that H3K4me3 is a solely a mark for activation and H3K9me3 marks silent heterochromatin domains.
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| |
|
| Overall design |
Samples consist of different strains, harvest times, and antibodies.
|
| |
|
| Contributor(s) |
J.Belden W, Zhu Q, Ramakrishnan M, Park J |
| Citation(s) |
31068130 |
| Submission date |
Oct 16, 2018 |
| Last update date |
May 21, 2019 |
| Contact name |
William J. Belden |
| E-mail(s) |
beldenwj@scarletmail.rutgers.edu
|
| Phone |
848-932-5617
|
| Organization name |
Rutgers, The State University of New Jersey
|
| Department |
Animal Sciences
|
| Lab |
Belden Lab
|
| Street address |
59 Dudley Rd
|
| City |
New Brunswick |
| State/province |
NJ |
| ZIP/Postal code |
08091 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20705 |
Illumina HiSeq 2500 (Neurospora crassa) |
|
| Samples (20)
|
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| This SubSeries is part of SuperSeries: |
| GSE121356 |
Context dependent Histone H3 Lysine 4 methylation is necessary for repression and is a requisite modification for facultative heterochromatin at distinct loci |
|
| Relations |
| BioProject |
PRJNA497093 |
| SRA |
SRP165901 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE121333_WT_DD_GSE121333_macs2_replicates_p_peaks.bed.gz |
18.5 Kb |
(ftp)(http) |
BED |
| GSE121333_WT_LP30_GSE121333_macs2_replicates_p_peaks.bed.gz |
18.8 Kb |
(ftp)(http) |
BED |
| GSE121333_macs2_K4_Dim5_DD_peaks.bed.gz |
134.6 Kb |
(ftp)(http) |
BED |
| GSE121333_macs2_K4_GSE121333_WT_DD_peaks.bed.gz |
124.4 Kb |
(ftp)(http) |
BED |
| GSE121333_macs2_K4_GSE121333_WT_LP30_peaks.bed.gz |
127.2 Kb |
(ftp)(http) |
BED |
| GSE121333_macs2_K4_dim5_LP30_peaks.bed.gz |
134.6 Kb |
(ftp)(http) |
BED |
| GSE121333_set1_DD_GSE121333_macs2_replicates_p_peaks.bed.gz |
22.0 Kb |
(ftp)(http) |
BED |
| GSE121333_set1_LP30_GSE121333_macs2_replicates_p_peaks.bed.gz |
20.8 Kb |
(ftp)(http) |
BED |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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