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| Status |
Public on Oct 26, 2018 |
| Title |
Expression data from the kidney of Sprague-Dawley rats given colistin methanesulfonate sodium |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the mechanism of nephrotoxicity, colistin methanesulfonate sodium (CMS; 25 or 50 mg/kg) was administered via intraperitoneal injection to Sprague–Dawley rats daily over 7 d.
Affymetrix GeneChip microarrays indicated 894 differentially expressed genes in groups treated with CMS (ANOVA, FDR < 0.05, fold change ≥ 1.3).
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| Overall design |
The Affymetrix GeneChip Rat 230 2.0 (Affymetrix, Santa Clara, CA, USA) was used for microarray analysis. We randomly selected three rats from each CMS-treated group (0, 25, and 50 mg/kg). Microarray analysis was conducted according to the GeneChip® 3' Expression Arrays User Guide (Affymetrix). cDNA synthesis, cRNA synthesis, biotin labeling, cRNA purification, and fragmentation were performed according to the manufacturer’s instructions (Affymetrix). The microarrays were hybridized at 45°C at 60 rpm for 16 h. The microarrays were stained and washed using a Fluidics Station 450 system (Affymetrix). A GeneChip® Scanner 3000 (Affymetrix) was used to scan the microarrays. The reproducibility of the microarray experiments was confirmed using well-defined quality control criteria according to the manufacturer’s instructions.
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| Contributor(s) |
Oh J, Yoon S |
| Citation(s) |
30628653 |
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| Submission date |
Oct 25, 2018 |
| Last update date |
Feb 15, 2019 |
| Contact name |
Jung-Hwa Oh |
| E-mail(s) |
jhoh@kitox.re.kr
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| Phone |
+82-42-610-+8278
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| Organization name |
Korea Institue of Toxicology
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| Street address |
141 Gajeong-ro, Yuseong
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| City |
Daejeon |
| ZIP/Postal code |
34114 |
| Country |
South Korea |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (9)
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| GSM3446242 |
Kidney at Colistin 50mg/kg for 7d, biological rep3 |
| GSM3446243 |
Kidney at Colistin 50mg/kg for 7d, biological rep2 |
| GSM3446244 |
Kidney at Colistin 50mg/kg for 7d, biological rep1 |
| GSM3446245 |
Kidney at Colistin 25mg/kg for 7d, biological rep3 |
| GSM3446246 |
Kidney at Colistin 25mg/kg for 7d, biological rep2 |
| GSM3446247 |
Kidney at Colistin 25mg/kg for 7d, biological rep1 |
| GSM3446248 |
Kidney at Colistin 0mg/kg for 7d, biological rep3 |
| GSM3446249 |
Kidney at Colistin 0mg/kg for 7d, biological rep2 |
| GSM3446250 |
Kidney at Colistin 0mg/kg for 7d, biological rep1 |
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| Relations |
| BioProject |
PRJNA498452 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE121792_RAW.tar |
23.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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