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Status |
Public on Oct 26, 2018 |
Title |
Genetic Study of anti-EGFR Antibody Therapy Response in Colorectal Carcinoma |
Organism |
Homo sapiens |
Experiment type |
Genome variation profiling by array
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Summary |
Metastatic colorectal carcinoma is one of the most common cancers in the world, second most commonly diagnosed cancer in Europe. Approximately 25% of patients initially diagnosed with colorectal carcinoma have metastases at the time of the first diagnosis and almost 50% of the diagnosed patients develop metastases, which explains the high mortality rate of these tumors. The carcinogenic process can be inhibited by the inactivation of EGFR receptors, making them important therapeutic targets. However, around 90-80% of patients do not respond to the EGFR targeted therapy. Resistance is mainly due to KRAS gene mutations. In this study, we built a 171 mutations panel (OncoAlvo®) that screens all mutations recommended by the ESMO, ASCO & NICE Medical Guidelines with 99% accuracy. The samples were simultaneously genotyped by other techniques to validate our panel and accuracy.
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Overall design |
457 human colorectal samples were genotyped for 171 somatic mutations (by the analysis of 61 positions) in 6 genes. 10 negative sample controls were used. 10 positive sample controls were used. All samples were genotyped by other technologies. The baseline threshold for the measure of the variants were set up with 96 normal samples.
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Contributor(s) |
Vazão H, Luís D, Caldas J, Antunes G, Freitas A, Santos S |
Citation missing |
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Submission date |
Oct 25, 2018 |
Last update date |
Oct 27, 2018 |
Contact name |
Helena Vazao |
Organization name |
HeartGenetics
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Department |
HeartGenetics
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Lab |
lab
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Street address |
Biocant Park Nucleo4, 4A
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City |
Cantanhede |
ZIP/Postal code |
3060-197 |
Country |
Portugal |
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Platforms (1) |
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Samples (553)
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Relations |
BioProject |
PRJNA498496 |
Supplementary file |
Size |
Download |
File type/resource |
GSE121805_G1104129.xml.gz |
2.6 Mb |
(ftp)(http) |
XML |
GSE121805_G1104129_processed.txt.gz |
2.3 Kb |
(ftp)(http) |
TXT |
GSE121805_G1104131.xml.gz |
2.6 Mb |
(ftp)(http) |
XML |
GSE121805_G1104148.xml.gz |
2.6 Mb |
(ftp)(http) |
XML |
GSE121805_G1116205.xml.gz |
1.3 Mb |
(ftp)(http) |
XML |
GSE121805_G1116205_processed.txt.gz |
1.6 Kb |
(ftp)(http) |
TXT |
GSE121805_G1116574.xml.gz |
2.7 Mb |
(ftp)(http) |
XML |
GSE121805_G1116574_processed.txt.gz |
2.5 Kb |
(ftp)(http) |
TXT |
GSE121805_G1116577.xml.gz |
2.6 Mb |
(ftp)(http) |
XML |
GSE121805_G1116577_processed.txt.gz |
2.6 Kb |
(ftp)(http) |
TXT |
GSE121805_G1129200.xml.gz |
2.7 Mb |
(ftp)(http) |
XML |
GSE121805_G1129200_processed.txt.gz |
2.6 Kb |
(ftp)(http) |
TXT |
GSE121805_G1129619.xml.gz |
2.6 Mb |
(ftp)(http) |
XML |
GSE121805_G1129619_processed.txt.gz |
2.7 Kb |
(ftp)(http) |
TXT |
GSE121805_G1135675.xml.gz |
2.7 Mb |
(ftp)(http) |
XML |
GSE121805_G1135675_processed.txt.gz |
2.9 Kb |
(ftp)(http) |
TXT |
GSE121805_G1135819.xml.gz |
2.8 Mb |
(ftp)(http) |
XML |
GSE121805_G1135819_processed.txt.gz |
2.1 Kb |
(ftp)(http) |
TXT |
GSE121805_G1135909.xml.gz |
2.7 Mb |
(ftp)(http) |
XML |
GSE121805_G1135909_processed.txt.gz |
2.7 Kb |
(ftp)(http) |
TXT |
GSE121805_G1139506.xml.gz |
2.7 Mb |
(ftp)(http) |
XML |
GSE121805_G1139506_processed.txt.gz |
2.3 Kb |
(ftp)(http) |
TXT |
GSE121805_G1140105.xml.gz |
2.0 Mb |
(ftp)(http) |
XML |
GSE121805_G1140105_processed.txt.gz |
1.4 Kb |
(ftp)(http) |
TXT |
GSE121805_G1156558.xml.gz |
381.0 Kb |
(ftp)(http) |
XML |
GSE121805_G1156558_processed.txt.gz |
1.3 Kb |
(ftp)(http) |
TXT |
GSE121805_Processed_Results_OncoAlvo.xlsx |
17.0 Kb |
(ftp)(http) |
XLSX |
GSE121805_RAW.tar |
10.0 Kb |
(http)(custom) |
TAR |
GSE121805_S0062445.xml.gz |
628.8 Kb |
(ftp)(http) |
XML |
GSE121805_S0062445_processed.txt.gz |
1.4 Kb |
(ftp)(http) |
TXT |
GSE121805_S0075587.xml.gz |
343.2 Kb |
(ftp)(http) |
XML |
GSE121805_S0075587_processed.txt.gz |
1.1 Kb |
(ftp)(http) |
TXT |
Processed data are available on Series record |