|
| Status |
Public on Jul 04, 2019 |
| Title |
siRNA knockdown in neonatal rat cardiac myocytes |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Primary neonatal rat cardiac myocytes were isolated and subjected to siRNA mediated Yap knockdown
|
| |
|
| Overall design |
Primary cardiac myocytes were isolated from two-day old rats via neonatal heart dissociation kit (Miltenyi Biotec) according to the manufacturer’s protocol followed by percoll gradient separation. Cells were transfected with siRNAs designed against a combination of Llgl1 and Llgl2, Yap, or universal negative control siRNAs. Transfected cells were collected 48 hours post transfection for RNAseq.
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| |
|
| Contributor(s) |
Flinn MA, O'meara CC, Link BA |
| Citation(s) |
32843528 |
| |
| Submission date |
Oct 29, 2018 |
| Last update date |
Aug 30, 2020 |
| Contact name |
Michael Andrew Flinn |
| Organization name |
Medical College of Wisconsin
|
| Department |
Physiology
|
| Lab |
O'Meara Lab
|
| Street address |
8701 W Watertown Plank Rd
|
| City |
Wauwatosa |
| State/province |
WI |
| ZIP/Postal code |
53226 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (9)
|
| GSM3450097 |
neonatal_rat_cardiomyocyte_negative control_1 |
| GSM3450098 |
neonatal_rat_cardiomyocyte_negative control_2 |
| GSM3450099 |
neonatal_rat_cardiomyocyte_negative control_3 |
| GSM3450100 |
neonatal_rat_cardiomyocyte_yap siRNA_1 |
| GSM3450101 |
neonatal_rat_cardiomyocyte_yap siRNA_2 |
| GSM3450102 |
neonatal_rat_cardiomyocyte_yap siRNA_3 |
|
| Relations |
| BioProject |
PRJNA499086 |
| SRA |
SRP167151 |
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