GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE122367 Query DataSets for GSE122367
Status Public on Aug 01, 2019
Title Gene expression profiling of basal and luminal epithelial cells from young adult and old mouse prostates
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We set out to determine if prostate epithelial cell-types exhibited age-related differences in their gene expression profiles. We used fluorescence activated cell sorting to isolate basal and luminal cells from dissociated preparations of prostate tissue taken from young adult (3 months) and old (24 months of age) mice. RNA was extracted from sorted cells to evaluate differences in gene expression. We found significant enrichment for progenitor genes in old luminal cells including an immune/inflammatory profile that overlapped with a previously identified CD38-lo human prostate luminal progenitor signature. This study demonstrates a luminal progenitor signature in old mouse prostate.
Overall design RNA sequencing of basal and luminal cells isolated from 3 month and 24 month old mouse prostates. Experiments were performed using biological triplicates, with each replicate pooled from 2 mice of the same age. Sequencing was performed on Illumina HiSeq 3000.
Contributor(s) Goldstein AS
Citation(s) 31390564, 32929410
Submission date Nov 09, 2018
Last update date Sep 21, 2020
Contact name Andrew Scott Goldstein
Organization name UCLA
Department Urology
Lab Goldstein
Street address 610 Charles E Young Dr East
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (12)
GSM3465075 Basal Young Rep 1
GSM3465076 Basal Young Rep 2
GSM3465077 Basal Young Rep 3
BioProject PRJNA504794
SRA SRP168352

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE122367_RAW.tar 5.1 Mb (http)(custom) TAR (of TXT)
GSE122367_TMM_normalized_reads_count_per_million.xlsx 3.2 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap