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| Status |
Public on Sep 22, 2020 |
| Title |
The epigenetic regulator Histone Deacetylase 3 regulates the ontogeny and maintenance of tissue-resident macrophage [scRNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Tissue-resident macrophages (TRMs) play central roles in local tissue development and immunity. However, how to control TRM ontogeny and maintenance remains unclear. We performed transcriptional and histone modification analyses of alveolar macrophages in mice with myeloid-specific (Csf1rCre) deletion of HDAC3 using bulk RNA-seq, single-cell RNA-seq and ChIP-seq. We report that HDAC3 deficiency results in metabolic disorders and increased cell death of the fetal lung TRMs, which is partially regulated through directly targeting PPAR-γ. Although the loss of AMs in the absence of HDAC3 is not AM subset specific, the transcriptome changes in HDAC3 deficient AM subsets are different. We propose that HDAC3 serves a key epigenetic regulator that controls embryonic TRM ontogeny and maintenance.
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| Overall design |
scRNA-sequencing of Csf1rCre+HDAC3fl/fl and Csf1rCre-HDAC3fl/fl adult lung alveolar macrophage
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| Contributor(s) |
Yao Y, Liu Q, Adrianto I, Glassbrook J, Wu X, Yin C, Wang Y, Zhang J, Peng H, Yang Y, Dong Z, Geissmann F, Zhou L, Mi Q |
| Citation(s) |
32732898 |
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| Submission date |
Nov 14, 2018 |
| Last update date |
Sep 22, 2020 |
| Contact name |
Qing-Sheng Mi |
| E-mail(s) |
qmi1@HFHS.ORG
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| Organization name |
Henry Ford Health System
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| Department |
Dermatology
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| Lab |
Immunology Program
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| Street address |
One Ford Place
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| City |
Detroit |
| State/province |
Michigan |
| ZIP/Postal code |
48202 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE122533 |
The epigenetic regulator Histone Deacetylase 3 regulates the ontogeny and maintenance of tissue-resident macrophage |
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| Relations |
| BioProject |
PRJNA505495 |
| SRA |
SRP168735 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE122529_RAW.tar |
504.3 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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