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Series GSE122741 Query DataSets for GSE122741
Status Public on Oct 01, 2019
Title The effects of Mkp-1 knockout and systemic E. coli infection on the transcriptome of the liver
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Previously, I have found that Mkp-1 knockout exacerbates inflammation, perturbs lipid metabolism, increases organ damage, mortality, and bacterial burdens. To understand the role of Mkp-1 in the regulation of host defense, we performed RNA-seq analysis using total RNA isolated from livers of uninfected and E. coli-infected wildtype and Mkp-1 knockout mice. Specifically, Mkp-1 wildtype and knockout (both on the C57/129 background) mice were infected with E. coli (O55:B5, ATCC 12014) at a dose of 2.5×107 CFU/g of body weight i.v., or given same volume of PBS. Mice were sacrificed 24 h post-infection to harvest organs. Livers were frozen clamping in liquid nitrogen, and stored at -80°C . Total RNA was isolated with Trizol and used for RNA-seq. RNA-seq results indicate that both E. coli infection and Mkp-1 knockout caused profound changes in the transcriptomes in the mouse livers.
Overall design Examination of the transcriptomal changes caused by E. coli sepsis in both Mkp-1 wildtype and knockout mice.
Contributor(s) Liu Y, Hafner M
Citation(s) 30563203, 32414764
Submission date Nov 20, 2018
Last update date Nov 09, 2020
Contact name Irina Buhimschi
Phone 3129968786
Organization name University of Illinois at Chicago College of Medicine
Department Department of Obstetrics & Gynecology
Lab Perinatal Research Laboratory
Street address 820 S. Wood St.
City Chicago
State/province IL
ZIP/Postal code 60612
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (16)
GSM3484447 Uninfected wildtype mouse (#1)
GSM3484448 Uninfected wildtype mouse (#2)
GSM3484449 Uninfected wildtype mouse (#6)
BioProject PRJNA506211
SRA SRP169858

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Supplementary file Size Download File type/resource
GSE122741_RAW.tar 2.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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