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| Status |
Public on Oct 01, 2019 |
| Title |
The effects of Mkp-1 knockout and systemic E. coli infection on the transcriptome of the liver |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Previously, I have found that Mkp-1 knockout exacerbates inflammation, perturbs lipid metabolism, increases organ damage, mortality, and bacterial burdens. To understand the role of Mkp-1 in the regulation of host defense, we performed RNA-seq analysis using total RNA isolated from livers of uninfected and E. coli-infected wildtype and Mkp-1 knockout mice. Specifically, Mkp-1 wildtype and knockout (both on the C57/129 background) mice were infected with E. coli (O55:B5, ATCC 12014) at a dose of 2.5×107 CFU/g of body weight i.v., or given same volume of PBS. Mice were sacrificed 24 h post-infection to harvest organs. Livers were frozen clamping in liquid nitrogen, and stored at -80°C . Total RNA was isolated with Trizol and used for RNA-seq. RNA-seq results indicate that both E. coli infection and Mkp-1 knockout caused profound changes in the transcriptomes in the mouse livers.
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| |
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| Overall design |
Examination of the transcriptomal changes caused by E. coli sepsis in both Mkp-1 wildtype and knockout mice.
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| Contributor(s) |
Liu Y, Hafner M |
| Citation(s) |
30563203, 32414764 |
| |
| Submission date |
Nov 20, 2018 |
| Last update date |
Nov 09, 2020 |
| Contact name |
Irina Buhimschi |
| E-mail(s) |
irina@uic.edu
|
| Phone |
3129968786
|
| Organization name |
University of Illinois at Chicago College of Medicine
|
| Department |
Department of Obstetrics & Gynecology
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| Lab |
Perinatal Research Laboratory
|
| Street address |
820 S. Wood St.
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60612 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA506211 |
| SRA |
SRP169858 |
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