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| Status |
Public on Mar 19, 2019 |
| Title |
AP-1cFos/JunB/miR-200a regulate the pro-regenerative glial cell response during axolotl spinal cord regeneration |
| Organism |
Ambystoma mexicanum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Salamanders have the remarkable ability to functionally regenerate after spinal cord transection. In response to injury, GFAP+ glial cells in the axolotl spinal cord proliferate and migrate to replace the missing neural tube and create a permissive environment for axon regeneration. Molecular pathways that regulate the pro-regenerative axolotl glial cell response are poorly understood. Here we show axolotl glial cells up-regulate AP-1cFos/JunB after injury, which promotes a pro-regenerative glial cell response. Axolotl glial cells directly repress c-Jun expression via up-regulation of miR-200a. Inhibition of miR-200a during regeneration causes defects in axonal regrowth and transcriptomic analysis revealed that miR-200a inhibition leads to differential regulation of genes involved with reactive gliosis, the glial scar, ECM remodeling and axon guidance. This work identifies a novel role for miR-200a in inhibiting reactive gliosis in glial cell in axolotl during spinal cord regeneration
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| Overall design |
All axolotls used in these experiments were bred in the axolotl facility at the University of Minnesota or at the MBL under the IACUC protocols #1710-35242A, #18-49. Axolotls of 2–3 cm were used for all in vivo experiments, and animals were kept in separate containers and fed daily with artemia; water was changed daily. Animals were anesthetized in 0.01% p-amino benzocaine (Sigma) before microinjection was performed.
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| Contributor(s) |
Sabin K, Jiang P, Gearhart M, Stewart R, Echeverri K |
| Citation(s) |
30854483 |
| |
| Submission date |
Nov 26, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Karen Echeverri |
| E-mail(s) |
kecheverri@mbl.edu
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| Organization name |
MARINE BIOLOGICAL LAB
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| Department |
Eugene Bell Center for Regeneration Biology and Tissue Engineering
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| Lab |
Echeverri Lab
|
| Street address |
7 MBL ST
|
| City |
Woods hole |
| State/province |
MA |
| ZIP/Postal code |
02543 |
| Country |
USA |
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| Platforms (1) |
| GPL22800 |
Illumina HiSeq 2500 (Ambystoma mexicanum) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA506973 |
| SRA |
SRP170730 |
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