|
| Status |
Public on Feb 11, 2019 |
| Title |
ChIP-seq analysis of transcription factor AtrR binding across the Aspergillus fumigatus genome |
| Organism |
Aspergillus fumigatus Af293 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To determine the genomic binding sites for the Aspergillus fumigatus transcription factor AtrR, we utilized three different strains. One was the wild-type AfS35 which contains a wild-type copy of atrR but lacks any HA tag. The other two are both derived from AfS35 but contain a 3X HA tag replacing the stop codon of atrR. These strains are SPF89 and SPF112, respectively. SPF89 uses the wild-type atrR promoter to drive expression of the epitope-tagged allele while SPF112 uses the much strong hspA promoter to drive production of the same protein.
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| |
|
| Overall design |
ChIP-seq using AtrR-3X HA expressed from either the wild-type or strong hspA promoter
|
| |
|
| Contributor(s) |
Paul S, Stamnes M, Moye-Rowley S |
| Citation(s) |
30862750 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI143198 |
Analysis of transcription factors determining azole resistance of Aspergillus fumigatus |
THE UNIVERSITY OF IOWA |
W Scott Moye-Rowley |
|
| |
| Submission date |
Nov 30, 2018 |
| Last update date |
Apr 02, 2019 |
| Contact name |
Scott Moye-Rowley |
| E-mail(s) |
scott-moye-rowley@uiowa.edu
|
| Phone |
3193357874
|
| Organization name |
University of Iowa
|
| Department |
Molecular Physiology & Biophysics
|
| Street address |
6-530 Bowen Science Bldg./Dept. of Mol Physio
|
| City |
Iowa City |
| State/province |
Iowa |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL25869 |
Illumina HiSeq 2500 (Aspergillus fumigatus Af293) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE123446 |
AtrR is an essential determinant of azole resistance in Aspergillus fumigatus |
|
| Relations |
| BioProject |
PRJNA507725 |
| SRA |
SRP171581 |
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