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| Status |
Public on Oct 27, 2008 |
| Title |
Expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation in vivo |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by array
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| Summary |
This study provides the first comprehensive analysis of gene expression and transcriptome dynamics of bovine metaphase II oocytes and in vivo developing bovine embryos.
For this study, Affymetrix GeneChip Bovine Genome Array which covers ~23,000 transcripts was used, which revealed several distinct clusters of genes regulated during various stages of bovine preimplantation development.
Keywords: Time course
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| Overall design |
In vivo matured oocytes were aspirated from the pre-ovulatory follicles of 9 animals on day 0 (=day of standing estrous). For embryo collection, donor animals were inseminated with frozen/thawed semen samples from one Holstein-Friesian bull with proven 270 fertility. Zygotes, 2-cell, 4-cell and 8-to 16-cell stages were recovered by oviductal flushing using a minimally invasive endoscopic approach (24 cows) 1-4 days after artificial insemination (AI). These oviductal stages were collected in pre-warmed phosphate buffered saline (PBS) containing 0.5% bovine serum albumin (BSA) (44). Morulae and blastocysts 12 were collected by uterine flushing at day 7 or 8 after AI (7 animals) using routine nonsurgical 275 procedures. All embryos were carefully evaluated under a stereo microscope and only morphologically intact embryos meeting established criteria (Manual of the International Embryo Transfer Society, IETS) were used. Embryos were pooled in groups of 5, in the case at blastocysts, or 10 embryos for the other stages, washed in PBS + 0.1% PVA, frozen in a minimum amount of medium in siliconised 0.6 ml cups and stored at -80°C. Typically, the 280 time period between flushing and freezing was less than 30 minutes. Animal treatments were conducted according to the German animal welfare guidelines.
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| Contributor(s) |
Sudheer S, Kues W, Niemann H, Adjaye J |
| Citation(s) |
19064908 |
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| Submission date |
Aug 04, 2008 |
| Last update date |
Dec 17, 2012 |
| Contact name |
Smita Sudheer |
| E-mail(s) |
sudheer@molgen.mpg.de, adjaye@molgen.mpg.de, kues@tzv.fal.de, niemann@tzv.fal.de
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| Organization name |
Max Planck Institute for Molecular Genetics
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| Street address |
Ihnestr. 63-73
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| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
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| Platforms (1) |
| GPL2112 |
[Bovine] Affymetrix Bovine Genome Array |
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| Samples (23)
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| GSM309704 |
Bovine Oocyte invitro, biological rep1 |
| GSM309705 |
Bovine Oocyte invitro, biological rep2 |
| GSM309706 |
Bovine Oocyte invitro, biological rep3 |
| GSM309707 |
Bovine Oocyte invivo, biological rep1 |
| GSM309708 |
Bovine Oocyte invivo, biological rep2 |
| GSM309709 |
Bovine Oocyte invivo, biological rep3 |
| GSM309710 |
Bovine Zygote, biological rep1 |
| GSM309711 |
Bovine Zygote, biological rep2 |
| GSM309712 |
Bovine Zygote, biological rep3 |
| GSM309713 |
Bovine 2 Cell, biological rep1 |
| GSM309714 |
Bovine 2 Cell, biological rep2 |
| GSM309715 |
Bovine 4 Cell, biological rep1 |
| GSM309716 |
Bovine 4 Cell, biological rep2 |
| GSM309717 |
Bovine 8 Cell, biological rep1 |
| GSM309718 |
Bovine 8 Cell, biological rep2 |
| GSM309719 |
Bovine 8 Cell, biological rep3 |
| GSM309720 |
Bovine 8 Cell, biological rep4 |
| GSM309721 |
Bovine Morula, biological rep1 |
| GSM309722 |
Bovine Morula, biological rep2 |
| GSM309723 |
Bovine Morula, biological rep3 |
| GSM309724 |
Bovine Blastocyst, biological rep1 |
| GSM309725 |
Bovine Blastocyst, biological rep2 |
| GSM309726 |
Bovine Blastocyst, biological rep3 |
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| Relations |
| BioProject |
PRJNA113561 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE12327_RAW.tar |
47.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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