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| Status |
Public on Feb 22, 2019 |
| Title |
Transcriptomic analysis of the Xrn1 depletion by Auxin-degron shutoff |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
The goal of the project was to study the effects on transcription and mRNA stability of the Xrn1 sudden depletion. We analyzed the effect of Xrn1 depletion caused by protein degradation of an Auxin-degron fusion on the transcription rates, mRNA stabilities and mRNA levels by doing Genomic Run-On (GRO) experiments at 30 min after Auxin addition with a control at 0 min.
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| Overall design |
Cells were grown in YPD at 30ºC to 0.5 OD600. At 0 min and 30 min after Auxin addition cells were spun-down and collected. Please note that each sample record represents three replicates (more details in the sample description field).
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| Contributor(s) |
Pérez-Ortín JE, García-Martínez J |
| Citation(s) |
30899024 |
| Submission date |
Dec 04, 2018 |
| Last update date |
Apr 02, 2019 |
| Contact name |
Jose E. Perez-Ortin |
| E-mail(s) |
jose.e.perez@uv.es
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| Phone |
34 963 543467
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| Organization name |
Universitat de Valencia
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| Department |
Bioquimica y Biologia Molecular
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| Lab |
Yeast Functional Genomics (GFL)
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| Street address |
Dr. Moliner 50
|
| City |
Burjassot |
| State/province |
Valencia |
| ZIP/Postal code |
E46100 |
| Country |
Spain |
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| Platforms (1) |
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| Samples (4)
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| GSM3500793 |
Processed RA data at time t30 after Auxin addition |
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| Relations |
| BioProject |
PRJNA508323 |
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