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| Status |
Public on Jun 21, 2019 |
| Title |
p63 cooperates with CTCF to modulate chromatin accessibility and architecture in skin keratinocytes |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Here we integrated multi-omics profiles including transcriptomics, DNA accessibility and capture Hi-C data to explore how p63 shapes local chromatin architecture in skin keratinocytes isolated from EEC syndrome patients. Surprisingly, we observed decreased chromatin accessibility in a number of DNA looping nodes which were co-mediated by p63 and CTCF. Our findings not only identified a new aspect of the bookmark function of p63, but also shed light on the disease mechanism underlined p63 dysfunction. Therefore, we propose p63 as a spatial genome organizer by modulating a subset of DNA loops with CTCF and therefore fine-tuning transcription programs required for skin keratinocytes.
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| |
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| Overall design |
ATAC-seq, CTCF ChIP-Seq of both control and p63 mutant keratinocytes on the proliferation stage
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| |
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| Contributor(s) |
QU J, YI G, ZHOU H |
| Citation(s) |
31164150 |
| |
| Submission date |
Dec 12, 2018 |
| Last update date |
Jun 14, 2022 |
| Contact name |
Jo Huiqing Zhou |
| E-mail(s) |
jo.zhou@radboudumc.nl
|
| Organization name |
Radboud University
|
| Street address |
Geert Grooteplein 26/28
|
| City |
Nijmegen |
| ZIP/Postal code |
6525GA |
| Country |
Netherlands |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA509627 |
| SRA |
SRP173326 |
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