GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE123783 Query DataSets for GSE123783
Status Public on Dec 14, 2018
Title The Neurospora crassa VE-1/VE-2/LAE-1 velvet complex controls development, secondary metabolism and light-dependent carotenoid biosynthesis
Organism Neurospora crassa
Experiment type Expression profiling by high throughput sequencing
Summary Neurospora crassa is a reference organism to study carotene biosynthesis and light regulation for decades. However, there is no evidence of its capacity to produce secondary metabolites, a characteristic of many filamentous fungi. In this work, we report the role of the fungal specific velvet regulator complex in development and secondary metabolism in N. crassa. Four velvet genes were found in the genome. Deletion of ve-1 or ve-2 affects asexual and sexual development, secondary metabolism and light-dependent carotene biosynthesis. Deletion of vos-1 did not show significant differences in comparison to wild type. Deletion of lae-1 resulted in reduced protoperithecia formation and affected secondary metabolism. VE-1, VE-2, LAE-1 and VOS-1 showed nucleo-cytoplasmic localization, which was independent of light input. Two distinct velvet complexes were found in vivo: a heterotrimeric VE-1/VE-2/LAE-1 and a heterodimeric VE-2/VOS-1 complex, respectively. The heterotrimer-complex positively regulated sexual development and repressed asexual spore formation. Moreover, it repressed siderophore coprogen production under iron starvation conditions. The VE-1/VE-2 heterodimer controlled the production of carotene pigments. VE-1 regulated the expression of more than 15% of the whole genome, which corresponded mainly to regulatory, developmental and redox proteins. We also studied intergenera functions of the velvet complex through complementation of A. nidulans veA, velB, laeA, vosA mutants with their Neurospora crassa orthologues ve-1, ve-2, lae-1 and vos-1, respectively. Expression of VE-1 and VE-2 in A. nidulans successfully substituted the developmental and secondary metabolite functions of VeA and VelB by forming two functional chimeric velvet complexes in vivo, VelB/VE-1/LaeA and VE-2/VeA/LaeA, respectively. The N. crassa lae-1 and vos-1 genes did not complement respective A. nidulans mutants and failed to form corresponding complexes. Reciprocally, expression of veA restored the phenotypes of the N. crassa ve-1 mutant. All N. crassa velvet proteins heterologously expressed in A. nidulans were predominantly localized to nuclear fraction independent of light signal. These data highlight the conservation of the complex formation potential in N. crassa and A. nidulans. However, they also underline the similarities and differences of the velvet roles across genera according to the different life styles, niches and ontogenetic processes.
Overall design Neurospora crassa wild-type or ve-1 duplicate cultures were incubated under standard and iron-free conditions for 48 hours and analyzed with polyA RNAseq.
Contributor(s) Bayram ÖS, Dettmann A, Karahoda B, Moloney N, Ormsby T, McGowan J, Cea-Sánchez S, Miralles-Durán A, Brancini GT, Fitzpatrick D, Cánovas D, Corrochano LM, Doyle S, Selker EU, Seiler S, Bayram Ö
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Dec 13, 2018
Last update date Dec 15, 2018
Contact name Tereza Ormsby
Organization name Charles University in Prague
Street address Hlavova 2030
City Prague 2
ZIP/Postal code 128 00
Country Czech Republic
Platforms (1)
GPL23150 Illumina HiSeq 4000 (Neurospora crassa)
Samples (8)
GSM3511336 wt_1_standcond
GSM3511337 wt_2_standcond
GSM3511338 ve-1_1_standcond
BioProject PRJNA509867
SRA SRP173377

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE123783_Dataset_1_stand_wt-v-ve1.xlsx 221.3 Kb (ftp)(http) XLSX
GSE123783_Dataset_2_Fefree_wt-v-ve1.xlsx 169.4 Kb (ftp)(http) XLSX
GSE123783_Dataset_3_wt_stand-v-Fefree.xlsx 136.6 Kb (ftp)(http) XLSX
GSE123783_Dataset_4_ve-1_stand-v-Fefree.xlsx 146.5 Kb (ftp)(http) XLSX
GSE123783_RAW.tar 320.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap