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| Status |
Public on Dec 19, 2018 |
| Title |
Response of Lactobacillus plantarum WCSF1 to the Gram-negative pathogen-associated quorum sensing molecule N-3-oxododecanoyl homoserine lactone. |
| Organism |
Lactiplantibacillus plantarum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The bacterial quorum sensing (QS) phenomenon has been well studied since its discovery and has traditionally been considered to include signaling pathways recognized exclusively within either Gram-positive (Gm+) or Gram-negative (Gm-) bacteria. The Gm+ bacteria are known to utilize quorum sensing pathways mediated by various small autoinducing peptides (AIP), while the Gm- bacteria use small molecules known collectively as acyl-homoserine lactones (AHL). Distinct structural differences between the types of signaling molecules have historically implied a lack of cross-talk among Gm+ and Gm- QS systems. Recent investigations, however, have demonstrated the ability for AIPs and AHLs to be produced by noncanonical organisms, implying QS systems may be more universally recognized than previously hypothesized. With that in mind, our interests were piqued by the organisms Lactobacillus plantarum, a Gm+ commensal probiotic known to participate in AIP-mediated QS, and Pseudomonas aeruginosa, a characterized Gm- pathogen whose virulence is controlled by AHL-mediated QS. Both health-related organisms are known to inhabit the human gut in various instances, both are characterized to elicit distinct effects on host immunity, and some studies hint at the putative ability of L. plantarum to degrade AHLs produced by P. aeruginosa. We therefore wanted to determine if L. plantarum cultures would respond to the addition of N-(3-oxododecanoyl)-L-homoserine lactone (3OC12) from P. aeruginosa by analyzing changes on both the transcriptome and proteome over time. Based on the observed upregulation of various two-component systems, response regulators, and native quorum sensing related genes, the resulting data provide evidence of an AHL recognition and response by L. plantarum.
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| Overall design |
L. plantarum was treated with 3OC12. Changes in the transcriptome were measured at three time points.
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| Contributor(s) |
Spangler JR, Dean SN, Leary DH, Walper SA |
| Citation(s) |
31024494 |
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Spangler JR, Dean SN, Leary DH, Walper SA. Response of Lactobacillus plantarum WCFS1 to the Gram-Negative Pathogen-Associated Quorum Sensing Molecule N-3-Oxododecanoyl Homoserine Lactone. Front. Microbiol. 2019;10:715. doi:10.3389/fmicb.2019.00715
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| Submission date |
Dec 18, 2018 |
| Last update date |
May 02, 2019 |
| Contact name |
Scott Nathan Dean |
| E-mail(s) |
scottnathandean@gmail.com
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| Organization name |
Naval Research Laboratory
|
| Street address |
4555 Overlook Ave SW
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| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20375 |
| Country |
USA |
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| Platforms (1) |
| GPL25957 |
Illumina MiSeq (Lactobacillus plantarum) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA510617 |
| SRA |
SRP173807 |
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