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| Status |
Public on Nov 11, 2020 |
| Title |
Human yolk sac-like haematopoiesis generates RUNX1-, GFI1- and/or GFI1B-dependent blood and SOX17-positive endothelium |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The genetic regulatory network controlling early fate choices during human blood cell development are not well understood. We use human pluripotent stem cell reporter lines to track the development of endothelial and haematopoietic populations in an in vitro model of human yolk-sac development. We identified SOX17-CD34+CD43- endothelial cells at day 2 of blast colony development, as a haemangioblast-like branch point from which SOX17-CD34+CD43+ blood cells and SOX17+CD34+CD43- endothelium subsequently arose. Most human blood cell development was dependent on RUNX1. Deletion of RUNX1 only permitted a single wave of yolk sac-like primitive erythropoiesis, but no yolk sac myelopoiesis or aorta-gonad-mesonephros (AGM)-like haematopoiesis. Blocking GFI1/1B activity with a small molecule inhibitor abrogated all blood cell development, even in cell lines with an intact RUNX1 gene. Together, our data defines the hierarchical requirements for both RUNX1 and GFI1/1B during early human haematopoiesis arising from a yolk sac-like SOX17- haemogenic endothelial intermediate.
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| |
|
| Overall design |
mRNA profiling of 16 samples for 6 different cell populations
|
| |
|
| Contributor(s) |
Bruveris FF, Elefanty AG |
| Citation(s) |
33028609 |
| |
| Submission date |
Dec 19, 2018 |
| Last update date |
Nov 11, 2020 |
| Contact name |
Freya Faith Bruveris |
| E-mail(s) |
freya.bruveris@mcri.edu.au
|
| Organization name |
Murdoch Children's Research Institute
|
| Department |
Cell Biology
|
| Lab |
Blood Development
|
| Street address |
50 Flemington Rd, Parkville
|
| City |
Melbourne |
| State/province |
VICTORIA |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (16)
|
|
| This SubSeries is part of SuperSeries: |
| GSE124086 |
Human yolk sac-like haematopoiesis generates RUNX1-, GFI1- and/or GFI1B-dependent blood and SOX17-positive endothelium |
|
| Relations |
| BioProject |
PRJNA510749 |
| SRA |
SRP173891 |
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