 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 19, 2019 |
| Title |
RAG1+ multipotent progenitors emerge directly from hemogenic endothelium of human PSC derived haemopoietic organoids [single cell RNA-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The Recombination Activation Gene, RAG1, expression of which presages T-cell receptor gene rearrangement, is a key marker of T-cell commitment. Using RAG1:GFP human pluripotent stem cell reporter lines, we examined human T-cells genesis in the context of haemtopoietic organoids. We show that T-cell commitment occurs concomitantly with the emergence of blood cells from AGM-like haemogenic endothelium, predating the surface expression of CD5 and CD7. In this system, RAG1 marks an early haematopoietic progenitor emerging from SOX17+ endothelium, prior to down regulation of CD90 and VCAM and upregulation of the blood cell marker, CD45. Sort and re-culture experiments show that early RAG1+ cells possess T-cell, B-cell, myeloid and erythroid potential. However, under conditions that favor T-cell development, early RAG1+ cells progress to the CD4+CD8+CD3+ stage, vindicating their classification as bone fide T-cell progenitors. These observations suggest that like the zebrafish and mouse, humans can execute a non-HSC derived wave of T-cell development that includes a multipotent RAG1+ progenitor.
|
| |
|
| Overall design |
sinlge cell transcriptional profiling of RAG1+ cells generated early on (day 20 and 16) during air-liquid interface culture of haemopoietic organoids
|
| |
|
| Contributor(s) |
Motazedian A, Stanley E |
| Citation(s) |
31907413 |
| |
| Submission date |
Dec 20, 2018 |
| Last update date |
Mar 08, 2020 |
| Contact name |
Ali Motazedian |
| E-mail(s) |
ali.motazedian@mcri.edu.au
|
| Organization name |
Murdoch Children's Research Institute
|
| Department |
Stem Cell Technology
|
| Street address |
Royal Children's Hospital
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA510945 |
| SRA |
SRP174020 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE124172_RAW.tar |
84.0 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
