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| Status |
Public on Dec 21, 2018 |
| Title |
Comparison of Porphyromonas gingivalis W83 gene expression when grown with or without galactose |
| Organism |
Porphyromonas gingivalis W83 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Porphyromonas gingivalis (P. gingivalis) W83 cultures in early exponential phase were supplemented with 0.1% galactose or vehicle control. The cultures were then permitted to grow anaerobically at 37°C for 3 hours, which corresponds to approximately one doubling of P. gingivalis. At this point, RNA extraction was performed using the cultures, and these samples were processed and submitted for RNA sequencing using an Illumina platform. Modest changes in gene expression were observed between cultures grown with or without galactose, and the majority of changes were associated with processes occurring at the cell membrane.
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| Overall design |
Cultures of P. gingivalis W83 were grown in Trypticase Soy Broth without glucose supplemented with hemin and menadione (TSBHK). Once cultures reached mid-exponential phase, the bacterial cultures were supplemented with 0.1% galactose or water (as a control). Both sets of cultures were grown in triplicate.
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| Contributor(s) |
Moye ZD, Gormley CM, Davey ME |
| Citation(s) |
30552185 |
| |
| Submission date |
Dec 20, 2018 |
| Last update date |
Jan 04, 2019 |
| Contact name |
Zachary Daniel Moye |
| Organization name |
University of Florida
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| Street address |
1395 Center Drive
|
| City |
Gainesville |
| State/province |
Florida |
| ZIP/Postal code |
32610 |
| Country |
USA |
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| Platforms (1) |
| GPL25969 |
Illumina HiSeq 3000 (Porphyromonas gingivalis W83) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA510987 |
| SRA |
SRP174073 |
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