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| Status |
Public on Dec 28, 2018 |
| Title |
Transcriptomic analysis of a caffeine-resistant Saccharomyces cerevisiae strain obtained by evolutionary engineering |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
A caffeine-resistant Saccharomyces cerevisiae mutant strain was obtained using an evolutionary engineering strategy based on successive batch cultivation at gradually increasing caffeine levels. The mutant strain Caf905-2 was selected at a caffeine concentration where its reference strain could not grow at all. Whole-genome transcriptomic analysis of Caf905-2 was performed with respect to its reference strain.
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| Overall design |
Agilent Yeast One-Color DNA microarray systems were employed for comparative whole-genome transcriptomic analysis of the selected mutant Caf905-2 and its reference strain. Before gene expression analysis, each culture was grown in triplicate in yeast minimal medium (YMM) at 30°C and 150 rpm. Total RNA was isolated, using RNeasy Mini Kit (Qiagen) at early exponential phase of growth (OD600 ~1.0).
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| Contributor(s) |
Sürmeli Y, Kısakesen HI, Çakar ZP |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 27, 2018 |
| Last update date |
Dec 30, 2018 |
| Contact name |
Halil ibrahim KISAKESEN |
| E-mail(s) |
kisakesenhi@gmail.com
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| Phone |
+905307831229
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| Organization name |
Istanbul Technical University
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| Department |
Molecular Biology, Genetics and Biotechnology
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| Lab |
Yeast
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| Street address |
ITU MOBGAM, SARIYER
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| City |
Istanbul |
| State/province |
Istanbul |
| ZIP/Postal code |
34469 |
| Country |
Turkey |
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| Platforms (1) |
| GPL16244 |
Agilent-016322 Yeast (V2) Gene Expression 8x15K Microarray (Probe Name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA512032 |
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