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| Status |
Public on Apr 28, 2021 |
| Title |
MYB ChIP-Seq of K562 cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We analyzed genome-wide chromatin binding of the transcription factor c-Myb using ChIP-Seq. K-562 cell lines stably expressing N-termianl 3xTY tagged Myb (pEFneo-3xTY-Myb) and control cell lines expressing the tag (pEFneo-3xTY) were used.
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| Overall design |
K-562 cells stably expressing pEFneo-3xTY-Myb and pooled control cell lines expressing pEFneo-TY were crosslinked followed by nuclei isolation and sonication. Sonicated chromatin was immunoprecipitated with a mouse monoclonal anti-TY1 antibody followed by elution of immnoprecipitated chromatin into two fractions. Pooled fractions were subjected to DNA purification using 'ChIP DNA clean concentrator KIT' (Zymo research #D5205). Libraries were prepared using ThruPLEXⓇ low-input sample-prep method. 75 bp single-end reads were generated from three biological replicates using the Illumina NextSeq 500 sequencer.
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| Contributor(s) |
Gabrielsen OS, Ledsaak M, Lemma RB |
| Citation(s) |
33903675 |
| |
| Submission date |
Jan 02, 2019 |
| Last update date |
Apr 28, 2021 |
| Contact name |
Roza Berhanu Lemma |
| E-mail(s) |
r.b.lemma@ibv.uio.no
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| Phone |
+4740975997
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| Organization name |
University of Oslo
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| Department |
Department of Biosciences
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| Lab |
Odd Stokke Gabrielsen
|
| Street address |
Blindernveien 31
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| City |
Oslo |
| ZIP/Postal code |
0371 |
| Country |
Norway |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA512464 |
| SRA |
SRP174998 |
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