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| Status |
Public on Jan 11, 2019 |
| Title |
Next generation sequencing (NGS) facilitates quantitative analysis of miRNA expression |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Using NGS approach we performed the search of multiple sclerosis-related miRNAs. We used PBMC as an informative and easily accessible biological material. To exclude bias in miRNA expression levels caused by disease modifying therapies, miRNA profiling was performed in treatment-naïve RRMS patients. Taking into account hypothetic gender specificity in disease pathogenesis we compared miRNA expression in RRMS patients and HCs separately for men and women. MiRNA profiling in men identified 32 differentially expressed miRNAs, which passed threshold for multiple corrections and may be attributed to MS-related. At the same time we did not find well-defined MS-specific miRNA expression signatures in women using NGS
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| Overall design |
We performed miRNA profiling in peripheral blood mononuclear cells of 16 relapsing-remitting MS (RRMS) patients and 8 healthy controls (HC).
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| Contributor(s) |
Baulina N, Kiselev I, Osmak G |
| Citation(s) |
30743997 |
| |
| Submission date |
Jan 10, 2019 |
| Last update date |
Mar 28, 2019 |
| Contact name |
Natalia Baulina |
| E-mail(s) |
tasha.baulina@gmail.com
|
| Phone |
+79852450645
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| Organization name |
Pirogov Russian National Research Medical University
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| Department |
Molecular biology and medical biotechnology
|
| Lab |
Medical genomics
|
| Street address |
Ostrovityanov 1
|
| City |
Moscow |
| ZIP/Postal code |
117997 |
| Country |
Russia |
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| Platforms (1) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA514238 |
| SRA |
SRP178219 |
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