|
| Status |
Public on Aug 01, 2019 |
| Title |
Sinorhizobium meliloti as a model system to study Ca. Liberibacter asiaticus regulators. [RpoH] |
| Platform organisms |
Sinorhizobium meliloti; Medicago truncatula |
| Sample organism |
Sinorhizobium meliloti |
| Experiment type |
Expression profiling by array
|
| Summary |
We characterized transcriptomes of a Sinorhizobium meliloti rpoH1rpoH2 deletion mutant (RFF231; Lang et al. 2018, mSphere 3:e00454-18) expressing either Ca. Liberibacter asiaticus rpoH or Sinorhizobium meliloti rpoH1
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| Overall design |
Gene expression profiling of Sinorhizobium meliloti strains was performed using custom Affymetrix GeneChips. Strain RFF231 (rpoH1rpoH2 deletion), carrying either Ca. Liberibacter asiaticus rpoH (locus tag = CLIBASIA_02490, codon optimized for expression in Sinorhizobium meliloti) or Sinorhizobium meliloti rpoH1 (locus tag = SMc00646) was compared to strain RFF231, carrying the empty vector, pSRKGm (Khan et al. 2008, Appl Environ Microbiol 74:5053-5062)
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| Contributor(s) |
Barnett MJ, Long SR |
| Citation(s) |
31427509 |
| |
| Submission date |
Jan 11, 2019 |
| Last update date |
Nov 04, 2019 |
| Contact name |
Melanie J Barnett |
| E-mail(s) |
melbar@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Biology
|
| Lab |
Sharon R. Long
|
| Street address |
371 Jane Stanford Way
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL9757 |
[stSmel_Mtra] SRL_SU Sinorhizobium meliloti/Medicago truncatula dual-genome SymbiosisChip, Affymetrix part number 510699 |
|
| Samples (9)
|
| GSM3560474 |
Sinorhizobium meliloti RFF231 pSRKGm, biological rep1 |
| GSM3560475 |
Sinorhizobium meliloti RFF231 pSRKGm, biological rep2 |
| GSM3560476 |
Sinorhizobium meliloti RFF231 pSRKGm, biological rep3 |
| GSM3560477 |
Sinorhizobium meliloti RFF231 pMB861, biological rep1 |
| GSM3560478 |
Sinorhizobium meliloti RFF231 pMB861, biological rep2 |
| GSM3560479 |
Sinorhizobium meliloti RFF231 pMB861, biological rep3 |
| GSM3560480 |
Sinorhizobium meliloti RFF231 pMB865, biological rep1 |
| GSM3560481 |
Sinorhizobium meliloti RFF231 pMB865, biological rep2 |
| GSM3560482 |
Sinorhizobium meliloti RFF231 pMB865, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE124984 |
Sinorhizobium meliloti as a model system to study Ca. Liberibacter asiaticus regulators. |
|
| Relations |
| BioProject |
PRJNA514735 |
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