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| Status |
Public on Sep 17, 2020 |
| Title |
TGF-b and Eomesodermin coordinate to maintain CD8+ regulatory T cells and limit spontaneous germinal center reaction |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We are interested in CD8+ regulatory T cells that limit germinal center reactions. We have found that in conditional knockout mice lacking both Tgfbr2 and Eomes expression in mature T cells, CD8+ regulatory T cells are severely defective. RNA-seq analysis was employed to characterize the genome-wide transcriptional changes in CD8+ regulatory T cells.
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| Overall design |
CD8+ regulatory T cells were FACS sorted from the spleens of 3-month old naïve WT and Tgfbr2-/-Eomes-/- mice. Total RNA was extracted and subjected to RNA-seq analysis.
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| Contributor(s) |
Mishra S, Ma C, Liu Y, Liao W, Lai Z, Zou Y, Chen Y, Zhang N |
| Citation(s) |
32991667 |
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| Submission date |
Jan 15, 2019 |
| Last update date |
Dec 17, 2020 |
| Contact name |
YI ZOU |
| E-mail(s) |
zou@uthscsa.edu
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| Organization name |
UTHSA-GCCRI
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| Street address |
8403 Floyd Curl Drive
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| City |
SAN ANTONIO |
| State/province |
TX |
| ZIP/Postal code |
78229 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA515265 |
| SRA |
SRP179612 |
