|
| Status |
Public on Jan 15, 2020 |
| Title |
RNA sequencing of host transcriptome of human foreskin fibroblast cells infected with Toxoplasma gondii bradyzoites, with and without myr1 (myc regulation 1) |
| Organisms |
Toxoplasma gondii; Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The purpose of these data is to determine if the impact bradyzoites have on the host transcripton is dependent on the export of parasite effector proteins via Myr1. We found that the expression of 3,068 host genes (~42%) were dependent on Myr1. A further 4,312 were altered in the same direction when comparing both the wild type and knock-out infected samples to the uninfected group. We can therefore conclude that Myr1 has an impact on the host transcriptional response to bradyzoite infection.
|
| |
|
| Overall design |
Five groups of human foreskin fibroblasts are compared which are either uninfected or infected with the bradyzoite stage of the Toxoplasma gondii parasite. These data consist of 15 samples, all taken from a single donor, with 3 samples per group. All samples have been incubated for 1 week in iM: RPMI-HEPES supplemented with 5% v/v fetal calf serum at pH 8.1-8.2. The samples are either uninfected host, wild-type (WT) bradyzoite infected, Myr1 knock-out (KO) bradyzoite infected, bystander WT bradyzoite, or bystander Myr1 KO bradyzoite. A bystander sample is a sample generated from cells that have been in the presence of the bradyzoite form of Toxoplasma but are not infected by it.
|
| |
|
| Contributor(s) |
Seizova S, Tonkin CJ, Garnham AL |
| Citation |
https://www.biorxiv.org/content/10.1101/669689v1
|
| |
| Submission date |
Jan 15, 2019 |
| Last update date |
Jan 19, 2020 |
| Contact name |
Alexandra Garnham |
| E-mail(s) |
garnham.a@wehi.edu.au
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Street address |
1G Royal Parade
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL26044 |
Illumina NextSeq 500 (Homo sapiens; Toxoplasma gondii) |
|
| Samples (15)
|
| GSM3563785 |
Uninfected host foreskin fibroblast replicate 1 |
| GSM3563786 |
Uninfected host foreskin fibroblast replicate 2 |
| GSM3563787 |
Uninfected host foreskin fibroblast replicate 3 |
| GSM3563788 |
Bystander bradyzoite (WT) foreskin fibroblast replicate 1 |
| GSM3563789 |
Bystander bradyzoite (WT) foreskin fibroblast replicate 2 |
| GSM3563790 |
Bystander bradyzoite (WT) foreskin fibroblast replicate 3 |
| GSM3563791 |
Bystander bradyzoite (Myr1 KO) foreskin fibroblast replicate 1 |
| GSM3563792 |
Bystander bradyzoite (Myr1 KO) foreskin fibroblast replicate 2 |
| GSM3563793 |
Bystander bradyzoite (Myr1 KO) foreskin fibroblast replicate 3 |
| GSM3563794 |
Infected bradyzoite (WT) foreskin fibroblast replicate 1 |
| GSM3563795 |
Infected bradyzoite (WT) foreskin fibroblast replicate 2 |
| GSM3563796 |
Infected bradyzoite (WT) foreskin fibroblast replicate 3 |
| GSM3563797 |
Infected bradyzoite (Myr1 KO) foreskin fibroblast replicate 1 |
| GSM3563798 |
Infected bradyzoite (Myr1 KO) foreskin fibroblast replicate 2 |
| GSM3563799 |
Infected bradyzoite (Myr1 KO) foreskin fibroblast replicate 3 |
|
| This SubSeries is part of SuperSeries: |
| GSE125122 |
Toxoplasma gondii bradyzoites manipulate host cell transcription |
|
| Relations |
| BioProject |
PRJNA515281 |
| SRA |
SRP179624 |
Less...
More...