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| Status |
Public on Jan 25, 2019 |
| Title |
RNAseq analysis of hematopoietic stem cells isolated from control and Regnase1 null mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The balance between self-renewal and differentiation of hematopoietic stem and progenitor cells (HSPCs) maintains hematopoietic homeostasis, failure of which can lead to hematopoietic disorder. HSPC fate is controlled by signals from the bone marrow niche resulting in alteration of the stem cell transcription network. Regnase-1, a member of the CCCH zinc finger protein family possessing RNAse activity, mediates post-transcriptional regulatory activity through degradation of target mRNAs. The precise function of Regnase-1 has been explored in inflammation-related cytokine expression but its function in hematopoiesis has not been elucidated. To clarify the role of Regnase-1 for hematopoiesis, we performed gene expression analysis on sorted HSC from control and Regnase1 null mice.
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| Overall design |
Bone marrow cells were obtained from femur of individual eight-week old Vav1-iCre; Reg1flox/flox mice (Reg1Δ/Δ, case) and control Reg1flox/flox mice (Reg1flox/flox ,control). RNAseq analyses were performed on HSCs (Lin- ScaI+ cKit+ CD34- Flt3- cells) purified by flow cytometry sorting.
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| Contributor(s) |
Kidoya H, Takakura N |
| Citation(s) |
30842549 |
| |
| Submission date |
Jan 24, 2019 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Hiroyasu Kidoya |
| Phone |
81-6-6879-8312
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| Organization name |
Osaka university
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| Department |
Research Institute for Microbial Diseases
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| Lab |
Department of Signal Transduction
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| Street address |
3-1 Yamadaoka
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| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA516795 |
| SRA |
SRP181804 |
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