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Series GSE125546 Query DataSets for GSE125546
Status Public on Jan 25, 2019
Title RNAseq analysis of hematopoietic stem cells isolated from control and Regnase1 null mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The balance between self-renewal and differentiation of hematopoietic stem and progenitor cells (HSPCs) maintains hematopoietic homeostasis, failure of which can lead to hematopoietic disorder. HSPC fate is controlled by signals from the bone marrow niche resulting in alteration of the stem cell transcription network. Regnase-1, a member of the CCCH zinc finger protein family possessing RNAse activity, mediates post-transcriptional regulatory activity through degradation of target mRNAs. The precise function of Regnase-1 has been explored in inflammation-related cytokine expression but its function in hematopoiesis has not been elucidated. To clarify the role of Regnase-1 for hematopoiesis, we performed gene expression analysis on sorted HSC from control and Regnase1 null mice.
Overall design Bone marrow cells were obtained from femur of individual eight-week old Vav1-iCre; Reg1flox/flox mice (Reg1Δ/Δ, case) and control Reg1flox/flox mice (Reg1flox/flox ,control). RNAseq analyses were performed on HSCs (Lin- ScaI+ cKit+ CD34- Flt3- cells) purified by flow cytometry sorting.
Contributor(s) Kidoya H, Takakura N
Citation(s) 30842549
Submission date Jan 24, 2019
Last update date Mar 21, 2019
Contact name Hiroyasu Kidoya
Phone 81-6-6879-8312
Organization name Osaka university
Department Research Institute for Microbial Diseases
Lab Department of Signal Transduction
Street address 3-1 Yamadaoka
City Suita
State/province Osaka
ZIP/Postal code 565-0871
Country Japan
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (10)
GSM3577024 Control_1
GSM3577025 Control_2
GSM3577026 Control_3
BioProject PRJNA516795
SRA SRP181804

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Supplementary file Size Download File type/resource
GSE125546_RAW.tar 23.8 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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