|
| Status |
Public on Jan 25, 2019 |
| Title |
microRNA responses of LPS and IL-10 stimulated primary human monocyte-derived macrophages over a short time-course |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Lipopolysaccharide stimulated primary human monocyte-derived macrophages at 2, 4 and 8hrs, IL-10 at 4hrs, IL-10+LPS at 4hrs
|
| |
|
| Overall design |
Dual label approach with each test sample was labelled with Hy3, and a “common reference” sample (obtained by combining equal quantities of RNA from each of the six experimental data points) was labelled with Hy5. Pooled donor approach: each test sample was pooled from 4 independent experiments with different human donors
|
| Web link |
https://doi.org/10.12688/wellcomeopenres.15065.1
|
| |
|
| Contributor(s) |
Simmonds RE, Feldmann M |
| Citation(s) |
31641696 |
| |
| Submission date |
Jan 24, 2019 |
| Last update date |
Oct 29, 2019 |
| Contact name |
RACHEL SIMMONDS |
| E-mail(s) |
rachel.simmonds@surrey.ac.uk
|
| Phone |
01483684714
|
| Organization name |
University of Surrey
|
| Street address |
FHMS UNIVERSITY OF SURREY, STAG HILL CAMPUS
|
| City |
GULDFORD |
| State/province |
Surrey |
| ZIP/Postal code |
GU2 7XH |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL7722 |
miRCURY LNA microRNA Array, v.10.0 - hsa, mmu & rno |
|
| Samples (6)
|
| GSM3577427 |
Primary macrophages 8hr stimulation with LPS |
| GSM3577428 |
Primary macrophages 4hr stimulation with LPS |
| GSM3577429 |
Primary macrophages 2hr stimulation with LPS |
| GSM3577430 |
Primary macrophages 4hr stimulation with IL-10 |
| GSM3577431 |
Primary macrophages 8hr stimulation with LPS & IL-10 |
| GSM3577432 |
Unstimulated primary macrophages (control) |
|
| Relations |
| BioProject |
PRJNA516857 |
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