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Series GSE125622 Query DataSets for GSE125622
Status Public on Jan 11, 2022
Title A Mediator-cohesin axis controls heterochromatin domain formation [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The genome consists of regions of transcriptionally active euchromatin and more silent heterochromatin. We reveal that the formation of heterochromatin domains requires cohesin turnover on DNA. Stabilization of cohesin on DNA through depletion of its release factor WAPL leads to a near-complete loss of heterochromatin domains. The Mediator CDK module controls heterochromatin in an opposing manner. Loss of this module leads to an almost binary partition of the genome into dense H3K9me3 domains, and regions devoid of H3K9me3 spanning the rest of the genome. By restricting the degree of heterochromatinization, the Mediator CDK module creates a transcription-permissive context that enables gene expression within heterochromatin domains. WAPL deficiency prevents the formation of heterochromatin domains, thus allowing for gene expression even in the absence of the Mediator CDK module. We propose that heterochromatin is controlled by two opposing activities, in which the Mediator CDK module and cohesin turnover must be balanced to enable the correct distribution of epigenetic marks to ensure proper gene expression.
Overall design ChIP-seq
Contributor(s) van der Weide RH, de Wit E
Citation(s) 35136067
Submission date Jan 24, 2019
Last update date Feb 23, 2022
Contact name Robin H. van der Weide
Organization name Hubrecht Institute
Lab Kind Lab
Street address Uppsalalaan 8
City Utrecht
State/province Utrecht
ZIP/Postal code 3584 CT
Country Netherlands
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (21)
GSM3579008 Hap1 WT H3K4me1 ChIPseq
GSM3579012 Hap1 WAPL/MED12 H3K4me1 ChIPseq
GSM3579014 Hap1 WAPL H3K4me1 ChIPseq
This SubSeries is part of SuperSeries:
GSE125672 A Mediator-cohesin axis controls heterochromatin domain formation.
BioProject PRJNA516928
SRA SRP181931

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Supplementary file Size Download File type/resource
GSE125622_RAW.tar 26.4 Mb (http)(custom) TAR (of BED, BROADPEAK, NARROWPEAK)
GSE125622_hap1_15_segments.bed.gz 2.7 Mb (ftp)(http) BED
GSE125622_hap1_med12_15_segments.bed.gz 2.1 Mb (ftp)(http) BED
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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