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| Status |
Public on Apr 30, 2019 |
| Title |
Chromatin immunoprecipitation sequencing (ChIP-Seq) for Bhlhe40 from naïve and IL-4c-stimulated large peritoneal macrophages (LPMs) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Most tissue-resident macrophage populations develop during embryogenesis, self-renew in the steady state, and can expand during type 2 immunity. Whether shared mechanisms regulate macrophage proliferation in homeostasis and disease is unclear. We found that the transcription factor Bhlhe40 was cell-intrinsically required in large peritoneal macrophages (LPMs) for self-renewal and maintenance, but was not required in other resident macrophages. Bhlhe40 was selectively necessary in LPMs for proliferation, but not polarization, in response to IL-4. During a helminth infection,
Bhlhe40 was required for normal LPM cell cycling. Bhlhe40 repressed the expression of Maf and Mafb and directly promoted expression of cell cycle-related transcripts to enable proliferation of LPMs. Genomic sites bound by Bhlhe40 in LPMs included some co-bound by the macrophage lineage-determining factor PU.1 and others uniquely bound by Bhlhe40, including Maf and cell cycle-related loci. Our findings demonstrate a tissue-specific control mechanism regulating resident macrophage proliferation in homeostasis and type 2 immunity.
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| Overall design |
LPMs were sorted from pooled naïve and pooled IL-4c-treated C57BL/6 mice. Chromatin was crosslinked and immunoprecipitation (Novus Biologicals, anti-Dec1, catalog NB100-1800, Lot C1) was performed for Bhlhe40-bound genomic regions, which were sequenced along with input DNA controls from the same samples.
DNA from 1 naïve LPM ChIP and 1 IL-4c-stimulated ChIP were sequenced, as well as input DNA. Cells were pooled from multiple mice for each immunoprecipitation.
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| Contributor(s) |
Jarjour NN, Shchukina I, Edelson BT |
| Citation(s) |
31061528 |
| |
| Submission date |
Jan 28, 2019 |
| Last update date |
Jul 30, 2019 |
| Contact name |
Brian T Edelson |
| E-mail(s) |
bedelson@path.wustl.edu
|
| Organization name |
Washington University in St. Louis
|
| Department |
Pathology and Immunology
|
| Lab |
Rm. 8304
|
| Street address |
425 S. Euclid Ave.
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE125730 |
Bhlhe40 mediates tissue-specific control of macrophage proliferation in homeostasis and type 2 immunity |
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| Relations |
| BioProject |
PRJNA517407 |
| SRA |
SRP182643 |
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